| 货号 | AF1268-SP |
| 别名 | A1A; A1AT; AATMGC23330; alpha 1-Antitrypsin; alpha 1-Proteinase Inhibitor; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; alpha-1-antitrypsin; antitrypsin), member 1; member 1; PIMGC9222; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; serine (or cysteine) proteinase inhibitor, clade A, member 1; Serpin A1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin) |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Simple Western(10 µg/mL) Immunoprecipitation(25 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Serpin A1/ alpha 1‑Antitrypsin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse (rm) Serpin A1A, recombinant human (rh) Serpin A3, rhSerpin A4, rhSerpin A5, rhSerpin A6, rhSerpin A7, rhSerpin A11, rhSerpin A12, rhSerpin C1, rhSerpin F2, and rmSerpin D1 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Simple Western: 10 µg/mL Immunoprecipitation: 25 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5265 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. S-glutathionylated serine proteinase inhibitors as plasma biomarkers in assessing response to redox-modulating drugs. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Serpin A1/ alpha 1‑Antitrypsin Glu25-Lys418 Accession # P01009 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Serpin A1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors (1). As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in the liver and secreted into the bloodstream with the major function to protect tissues against neutrophil elastase. A severe serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma (2). The deficiency is caused by point mutations in naturally occurring serpin A1 variants (over 70 are known). For example, the Z variant (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe serpin A1 deficiency (3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Serpin A1/ alpha 1‑Antitrypsin by Western Blot. Western blot shows human plasma and lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Serpin A1/ alpha 1‑Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Serpin A1/ alpha 1‑Antitrypsin in HepG2 Human Cell Line. Serpin A1/ alpha 1‑Antitrypsin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Serpin A1/ alpha 1‑Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human Serpin A1/ alpha 1‑Antitrypsin by Simple WesternTM. Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 62 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Serpin A1/ alpha 1‑Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. | |
Detection of Human Serpin A1/ alpha 1‑Antitrypsin by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/ alpha 1‑Antitrypsin at approximately 65 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Serpin A1/ alpha 1‑Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1268). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
