| 货号 | AF1254-SP |
| 别名 | beta-Netrin; CDY; CDY1A; chromodomain protein, Y chromosome, 1; chromodomain protein, Y-linked, 1; FLJ77185; NTN4; testis-specific chromodomain protein on Y; testis-specific chromodomain protein Y 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Netrin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Netrin-4 is observed, 15% cross-reactivity with recombinant chicken (rch) Netrin-1 is observed and less than 5% cross‑reactivity with recombinant mouse (rm) Netrin-G1a, Netrin-G2a, and rchNetrin-2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 59277 (Human); 57764 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Identification of NTN4, TRA1, and STC2 as prognostic markers in breast cancer in a screen for signal sequence encoding proteins. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Netrin-4 Val26-Lys628 Accession # Q9HB63 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins bind to the DCC and UNC5 family of receptors to attract or repel axons. Human Netrin-4 is synthesized as a 628 amino acid (aa) precursor that contains a 19 aa signal sequence, a 443 aa laminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 166 aa C‑terminal domain rich in basic aa residues that serves as a heparin binding site. Unlike human Netrin-1 which is reminiscent of the laminin gamma -chain, human Netrin-4 resembles the laminin beta -chain. Netrin-4 has been reported to exist as both a monomer and a dimer. The dimeric form was reported to be the less active of the two. Human Netrin-4 shares 31%, 29% and 25% aa sequence identity with human Netrin-1, 2 and G2, respectively; it shares 89% aa identity with mouse Netrin-4. Netrin-4 has widespread expression, occurring in nervous tissues such as embryonic floor plate and postnatal neurons such as cerebellar granule cells and hippocampal pyramidal cells. It also is found in non-neural tissues, such as adult Bowman’s capsule and medullary tubular epithelium in kidney, and embryonic pancreatic and intestinal epithelium, plus cells of the ureteric bud. Netrin-4 expression is often associated with basement membrane. Netrin-4 has been shown to initiate axon outgrowth from olfactory bulb explants (1‑7). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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