Human/Mouse/Rat MDM2/HDM2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse/Rat MDM2/HDM2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1244-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1244-SP
别名	ACTFS; HDM2; HDMX; MDM2 oncogene, E3 ubiquitin protein ligase
全称	Double Minute 2 Protein
反应种属	Human/Mouse/Rat
应用	Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human, mouse, and rat MDM2.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4193 (Human); 17246 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 (cis-imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas. J Hematol Oncol, 2012;5(0):57. Species: Human Sample Type: Cell Lysates Application: WB Cell death via DR5, but not DR4, is regulated by p53 in myeloma cells. Authors: Surget S, Chiron D, Gomez-Bougie P, Descamps G, Menoret E, Bataille R, Moreau P, Le Gouill S, Amiot M, Pellat-Deceunynck C Cancer Res, 2012;72(17):4562-73. Species: Human Sample Type: Cell Lysates Application: WB HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 (cis-imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas. Authors: Sosin, Angela M, Burger, Angelika, Siddiqi, Aisha, Abrams, Judith, Mohammad, Ramzi M, Al-Katib, Ayad M J Hematol Oncol, 2012;5(0):57. Species: Human Sample Type: Cell Lysates Application: WB A high-frequency regulatory polymorphism in the p53 pathway accelerates tumor development. Authors: Post SM, Quintas-Cardama A, Pant V, Iwakuma T, Hamir A, Jackson JG, Maccio DR, Bond GL, Johnson DG, Levine AJ, Lozano G Cancer Cell, 2010;18(3):220-30. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded CARPs are ubiquitin ligases that promote MDM2-independent p53 and phospho-p53ser20 degradation. Authors: Rozan LM, McDonald ER, Navaraj A, Matthew EM, Dicker DT, El-Deiry WS J. Biol. Chem., 2007;282(5):3273-81. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human MDM2 aa 3-491
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	MDM2 is a key regulator of p53 tumor suppressor protein activity and stability. MDM2 binds to and inhibits the transactivation domain of p53. In addition, MDM2 controls p53 stability by functioning as its E3 ligase in ubiquitination and by shuttling p53 from the nucleus to the cytoplasm for subsequent degradation. The importance of the p53/MDM2 relationship is underscored by the existence of an autoregulatory feedback loop whereby activated p53 transcriptionally up‑regulates the expression of its own inhibitor, MDM2.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers Checkpoint Chemokine Receptor Intracellular Signaling E3 Ubiquitin Ligases ICC / IHC Images: MDM2/HDM2 Notch Pathway p53 Pathway Ubiquitin-related Molecules in the Akt Pathway

参考图片

MDM2 in Mouse Kidney. MDM2 was detected in perfusion fixed frozen sections of mouse kidney using Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human MDM2 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, MCF‑7 human breast cancer cell line, and CEM human T‑lymphoblastoid cell line untreated (-) or treated (+) with 5 μM LLnV for 1 hour. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MDM2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.