Human CD23/Fc epsilon RII Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human CD23/Fc epsilon RII Affinity Purified Polyclonal Ab (25 UG)

货号： AF123-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF123-SP
别名	BLAST-2; CD23; CD23A; CD23CD23 antigen; CLEC4J; CLEC4JC-type lectin domain family 4 member J; C-type lectin domain family 4, member J; Fc epsilon RII; Fc fragment of IgE, low affinity II, receptor for (CD23); FCE2Fc fragment of IgE, low affinity II, receptor for (CD23A); fc-epsilon-RII; FCER2; Fcer2a; IGEBF; Immunoglobulin E-binding factor; low affinity immunoglobulin epsilon Fc receptor; Ly-42; Lymphocyte IgE receptor
全称	Fc epsilon Receptor II
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human CD23/Fc epsilon RII in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.5-2.5 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of human IgE to immobilized Recombinant Human CD23/Fc epsilon RII (Catalog # 123-FE) coated at 2 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding. Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2208 (Human); 14128 (Mouse); 171075 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cytoplasmic myosin-exposed apoptotic cells appear with caspase-3 activation and enhance CLL cell viability. Authors: Cui X, Zhang L, Magli A, Catera R, Yan X, Griffin D, Rothstein T, Barrientos J, Kolitz J, Allen S, Rai K, Chiorazzi N, Chu C Leukemia, 2016;30(1):74-85. Species: Human Sample Type: Whole Cells Application: IHC - Not specified
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human CD23/Fc epsilon RII Met150-Ser321 Accession # P06734
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CD23 (also named B cell differentiation antigen) is a member of subgroup II of the C-type (Ca⁺⁺-dependent) lectin superfamily (1-5). Human CD23 is a 47 kDa, type II transmembrane glycoprotein that is expressed by a wide variety of cell types (6-10). The full-length receptor is 321 amino acids (aa) in length and contains a 274 aa extracellular region, a 26 aa transmembrane segment, and a 21 aa cytoplasmic domain. The extracellular region contains a C-type lectin domain and a connecting stalk with coiled-coil topography (3, 11). The lectin domain binds both protein and carbohydrate in an apparently Ca⁺⁺ independent manner (11). The coiled-coil region contributes to oligomerization (11, 12). The lectin domain in human CD23 (aa 162-284) shares 64%, 62%, and 68% aa sequence identity with the lectin domains in mouse, rat, and bovine CD23, respectively. In the cytoplasmic region, two FC isoforms exist which arise from alternate start sites (6, 12). The “a” (or long) isoform begins with the sequence MEEGQYS and is constitutively expressed by B cells. It is believed to participate in IgE-mediated endocytosis (13). The “b” (or short) isoform begins with MNPPSQ and is induced on a wide variety of cell types by IL-4 (6). Fcb reportedly contributes to IgE-mediated phagocytosis (13). Fcb expressing cells include eosinophils, monocytes, visceral smooth muscle and intestinal epithelium (6, 14, 15). At least four soluble forms of CD23 are known to exist. They range in molecular weight from 25 kDa to 37 kDa, with the 25 kDa form predominating in sera (16). Soluble CD23 (sFc) is generated by metalloprotease (ADAM8; ADAM15; ADAM28) and cysteine-protease activity (16-18). Cleavage usually occurs between aa 150-160 (7, 8). It is unclear if sequential metalloprotease-cysteine protease activity is necessary for the generation of all soluble forms. Both soluble and membrane-bound CD23 show bioactivity. Ligands for CD23 include CD21, IgE, CD11b, and CD11c (19-21). CD23 binding to CD11b and Cd11c on monocytes results in oxidative product generation and proinflammatory cytokine release (21). On B cells, sCD23 induces IgE secretion by binding CD21. Conversely, secreted IgE will, in turn, bind B cell membrane CD23, rendering it unavailable for cleavage, and thus shutting down IgE production (11).
运输条件	Blue Ice
存放说明	4℃
参考文献	Kijimoto-Ochiai, S. (2002) Cell. Mol. Life Sci. 59:648. Heyman, B. (2000) Annu. Rev. Immunol. 18:709. Bajorath, J. and A. Aruffo (1996) Protein Sci. 5:240. Drickamer, K. (1993) Curr. Opin. Struct. Biol. 3:393. Drickamer, K. (1999) Curr. Opin. Struct. Biol. 9:585. Yokota, A. et al. (1988) Cell 55:611. Ludin, C. et al. (1987) EMBO J. 6:109. Ikuta, K. et al. (1987) Proc. Natl. Acad. Sci. USA 84:819. Kikutani, H. et al. (1986) Cell 47:657. Letellier, M. et al. (1988) J. Immunol. 141:2374. Hibbert, R.G. et al. (2005) J. Exp. Med. 202:751. Beavuil, A.J. et al. (1992) Proc. Natl. Acad. Sci. USA 89:753. Yokota, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:5030. Belleau, J.T. et al. (2005) Clin. Mol. Allergy 3:6. Tu, Y. et al. (2005) Gastroenterology 129:928. Marolewski, A.E. et al. (1998) Biochem. J. 333:573. Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469. Karagiannis, S.N. et al. (2001) Immunology 103:319. Aubry, J-P. et al. (1992) Nature 358:505. Sarfati, M. and G. Delespeese (1988) J. Immunol. 141:2195. Lecoanet-Henchoz, S. et al. (1995) Immunity 3:119.

参考图片

CD23/Fc epsilon RII in Human PBMCs. CD23/Fc epsilon RII was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human CD23/Fc epsilon RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF123) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.