Human Integrin alpha V/CD51 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Integrin alpha V/CD51 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1219-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1219-SP
别名	antigen identified by monoclonal L230; CD51 antigen; CD51; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITGAV; MSK8; Vitronectin Receptor alpha; Vitronectin receptor subunit alpha; VNRA; VNRADKFZp686A08142
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human Integrin alpha V in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant human Integrin alpha 5 and recombinant mouse Integrin E is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3685 (Human); 16410 (Mouse); 296456 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Characterization of human and murine T-cell immunoglobulin mucin domain 4 (TIM-4) IgV domain residues critical for Ebola virus entry J Virol, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Flow Quantity and accessibility for specific targeting of receptors in tumours. Authors: Hussain, Sajid, Rodriguez-Fernandez, Maria, Braun, Gary B, Doyle, Francis, Ruoslahti, Erkki Sci Rep, 2014;4(0):5232. Species: Human Sample Type: cell line Application: IF Binding of extracellular maspin to beta1 integrins inhibits vascular smooth muscle cell migration. Authors: Bass R, Wagstaff L, Ravenhill L, Ellis V J. Biol. Chem., 2009;284(40):27712-20. Species: Human Sample Type: Cell Lysates Application: IP
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha V/CD51 Phe31-Val992 Accession # P06756
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Integrin alpha V, also known as CD51 and vitronectin receptor subunit alpha, is a 140-150 kDa integrin alpha chain that forms dimers with at least five beta chains including beta 1, 3, 5, 6, and 8. It is a 1018 amino acid (aa) residue type I membrane protein with a large (962 aa) extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The N-terminal region of alpha V, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) concensus repeats that fold into a beta -propellar domain. Furin cleavage of the alpha V ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2, and TSP. The ECD of human Integrin alpha V shares 92% aa sequence identity with mouse Integrin alpha V ECD.
运输条件	Blue Ice
存放说明	4℃
参考文献	Actin Stress Fibers and Focal Adhesions During EMT Adhesion Molecules Endothelial Cell Markers and Adhesion Molecules in Angiogenesis Epithelial Cell Polarity HGF/SF Activation of Integrins Human CD Marker Products ICC / IHC Images: Integrin alpha V/CD51 Integrin Ligands at a Glance (J. Cell. Sci.) Integrin Subunit Interactions Integrins and Associated Molecules Mesenchymal Stem Cell Markers Osteoblast and Osteoclast Markers VEGF Family

参考图片

Integrin alpha V/CD51 in Rat Mesenchymal Stem Cells. Integrin alpha V/CD51 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Human Integrin alpha V/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.