| 货号 | AF1207-SP |
| 别名 | CRL4IL-17 receptor homolog 1; cytokine receptor CRL4; Cytokine receptor-like 4; Evi27; EVI27IL-17B receptor; IL-17 receptor B; IL-17 RH1; IL17BRinterleukin 17B receptor; IL-17ER; IL17RB; IL-17RB; IL17Rh1; IL-17Rh1; IL17RH1MGC5245; interleukin 17 receptor B; interleukin 17 receptor homolog 1; interleukin-17 receptor B; Interleukin-17B receptor | 全称 | Interleukin 17B Receptor |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human IL‑17 RB in Western blots. In Western blots, approximately 35% cross-reactivity with recombinant mouse (rm) IL‑17 RB is observed, approximately 5% cross-reactivity with rmIL-17 R is observed, and less than 1% cross-reactivity with recombinant human IL‑17 R is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 55540 (Human); 50905 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Interleukin-25-induced chemokines and interleukin-6 release from eosinophils is mediated by p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, and nuclear factor-kappaB. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human IL‑17 RB Arg18-Gly289 Accession # NP_061195 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These pro-inflammatory cytokines can induce local cytokine productions and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1). Interleukin-17 B Receptor (IL-17B R), also known as IL-17Rh1, IL-17E R. and EVI27, represents the second receptor of the IL-17 family of cytokines to be recognized (2-4). Human IL-17B R cDNA encodes a 502 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 275 aa extracellular domain, a 21 aa transmembrane domain, and a 189 aa cytoplasmic tail. By alternative splicing, a secreted variant of IL-17B R has also been identified (4). Human and mouse IL‑17B R share 76% aa sequence identity. The human IL-17B R protein sequence is only 19.2% identical to the human IL-17 R sequence, but the two receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. Three additional type I transmembrane receptors with homology to IL-17 R have been reported, increasing the number of the IL-17 R family members to five (5, 6). By Northern blot analysis, human IL-17B R is highly expressed in kidneys and liver but is expressed at lower levels in testes, brain, small intestine and other endocrine tissues (2-4). The expression of IL-17B R is significantly up-regulated under inflammatory conditions. IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17, IL-17C, and IL-17F. Activation of IL-17B R by its ligands results in the activation of nuclear factor kappa-B (2-4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IL‑17 RB in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human IL‑17 RB Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1207, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). | |
IL‑17 RB in Human Kidney. IL‑17 RB was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Goat Anti-Human IL‑17 RB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1207) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
