Human BAFF R/TNFRSF13C Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human BAFF R/TNFRSF13C Affinity Purified Polyclonal Ab (25 UG)

货号： AF1162-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1162-SP
别名	BR3; CD268; TNFRSF13C
全称	B cell Activating Factor Receptor
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects human BAFF R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse BAFF R and less than 2% cross-reactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhNGF R, rhOPG, rhRANK, rhTNF RI, and rhTNF RII is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Neutralization: Measured by its ability to neutralize BAFF R/TNFRSF13C-mediated inhibition of proliferation in mouse B cells. The Neutralization Dose (ND₅₀) is typically 3-15 µg/mL in the presence of 0.3 µg/mL Recombinant Human BAFF R/TNFRSF13C Fc Chimera and 5 ng/mL Recombinant Human BAFF/BLyS/TNFSF13B.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	115650 (Human); 72049 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F Leukemia, 2016;30(1):163-72. Species: Human Sample Type: Whole Cells Application: Neut BAFF levels are increased in lesional skin and sera in patients with cutaneous T-cell lymphoma. Authors: Ohmatsu H, Sugaya M, Miyagaki T, Suga H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S Br. J. Dermatol., 2012;167(2):359-67. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Fusion Toxin BLyS-Gelonin Inhibits Growth of Malignant Human B Cell Lines In Vitro and In Vivo. Authors: Luster, Troy A, Mukherjee, Ipsita, Carrell, Jeffrey, Cho, Yun Hee, Gill, Jeffrey, Kelly, Lizbeth, Garcia, Andy, Ward, Christop, Oh, Luke, Ullrich, Stephen, Migone, Thi-Sau, Humphreys, Robin PLoS ONE, 2012;7(10):e47361. Species: Human Sample Type: Whole Cells Application: Neut APRIL Binding to BCMA Activates a JNK2-FOXO3-GADD45 Pathway and Induces a G2/M Cell Growth Arrest in Liver Cells. Authors: Notas G, Alexaki V, Kampa M, Pelekanou V, Charalampopoulos I, Sabour-Alaoui S, Pediaditakis I, Dessirier V, Gravanis A, Stathopoulos E, Tsapis A, Castanas E J Immunol, 2012;189(10):4748-58. Species: Human Sample Type: Whole Cells Application: ICC Requirement for BAFF and APRIL during B cell development in GALT. Authors: Yeramilli VA, Knight KL J. Immunol., 2010;184(10):5527-36. Species: Human Sample Type: Whole Cells Application: Flow BAFF enhances chemotaxis of primary human B cells: a particular synergy between BAFF and CXCL13 on memory B cells. Authors: Badr G, Borhis G, Lefevre EA, Chaoul N, Deshayes F, Dessirier V, Lapree G, Tsapis A, Richard Y Blood, 2008;111(5):2744-54. Species: Human Sample Type: Whole Cells Application: Flow Neutralizing B-cell activating factor antibody improves survival and inhibits osteoclastogenesis in a severe combined immunodeficient human multiple myeloma model. Authors: Neri P, Kumar S, Fulciniti MT, Vallet S, Chhetri S, Mukherjee S, Tai Y, Chauhan D, Tassone P, Venuta S, Munshi NC, Hideshima T, Anderson KC, Raje N Clin. Cancer Res., 2007;13(19):5903-9. Species: Human Sample Type: Whole Cells Application: Flow IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells. Authors: Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE J. Immunol., 2007;178(5):2872-82. Species: Human Sample Type: Whole Cells Application: Flow B-lymphocyte stimulator (BLyS) stimulates immunoglobulin production and malignant B-cell growth in Waldenstrom macroglobulinemia. Authors: Elsawa SF, Novak AJ, Grote DM, Ziesmer SC, Witzig TE, Kyle RA, Dillon SR, Harder B, Gross JA, Ansell SM Blood, 2006;107(7):2882-8. Species: Human Sample Type: Whole Cells Application: Flow Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma. Authors: Pound JD, Batth BK, Johannessen I, Wood K J. Immunol., 2005;174(5):3015-23. Species: Human Sample Type: Whole Cells Application: Neut Decreased survival of B cells of HIV-viremic patients mediated by altered expression of receptors of the TNF superfamily. Authors: Moir S, Malaspina A, Pickeral OK, Donoghue ET, Vasquez J, Miller NJ, Krishnan SR, Planta MA, Turney JF, Justement JS, Kottilil S, Dybul M, Mican JM, Kovacs C, Chun TW, Birse CE, Fauci AS J. Exp. Med., 2004;200(7):587-99. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human BAFF R/TNFRSF13C Arg2-Ala71 Accession # Q96RJ3
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	B cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation (1-4). BAFF binds to three TNF receptor superfamily members: B cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4⁺T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells. Similarly, A/WySnJ mice that are defective in BAFF-R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B cell development. While the function of BCMA is not defined, TACI has been shown to control B cell homeostasis and T cell-independent immune responses.
运输条件	Blue Ice
存放说明	4℃
参考文献	Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266. Mackay F. and J.L. Browning (2002) Nature Reviews Immunology 2:464. Laabi, Y. et al. (2001) Current Biol. 11:R1013. Thompson, J.S. et al. (2001) Science 14:2108.

参考图片

Detection of BAFF R/TNFRSF13C in PBMCs by Flow Cytometry. Peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Goat Anti-Human BAFF R/TNFRSF13C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1162) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by APC-conjugated anti-goat secondary antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

BAFF R/TNFRSF13C Inhibition of BAFF/BLys/
TNFSF13B-dependent Cell Proliferation and Neutralization by Human BAFF R/TNFRSF13C Antibody. Recombinant Human BAFF R/TNFRSF13C Fc Chimera (Catalog #
1162‑BR) inhibits Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149‑BF) induced proliferation in mouse B cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human BAFF/BLyS/TNFSF13B (5 ng/mL) activity elicited by Recombinant Human BAFF R/TNFRSF13C Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti‑Human BAFF R/TNFRSF13C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1162). The ND₅₀ is typically
3-15 µg/mL.