| 货号 | AF1148-SP |
| 别名 | coagulation factor II (thrombin) receptor-like 2; Coagulation factor II receptor-like 2 (protease-actovated receptor 3); Coagulation factor II receptor-like 2; Coagulation Factor II; F2; PAR-3; PAR3proteinase-activated receptor 3; protease-activated receptor 3; proteinase-activated receptor-3; PT; serine protease; Thrombin receptor-like 2; Thrombin |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Simple Western(10 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human Coagulation Factor II/Thrombin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) PROC is observed, and less than 1% cross-reactivity with rhFactor X, recombinant mouse (rm) Factor X and rmPROC is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Simple Western: 10 µg/mL Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2147 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Protease-activated receptor 2 blocking peptide counteracts endotoxin-induced inflammation and coagulation and ameliorates renal fibrin deposition in a rat model of acute renal failure. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor II/Thrombin |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Coagulation Factor II, commonly known as thrombin, is an essential component of the coagulation cascade in which it converts fibrinogen to fibrin, activates factors V, VII, VIII, XIII and forms complexes with protein C and thrombomodulin (1). It also activates platelets and regulates the behavior of additional cells through protease‑activated receptors (PARs) (2). It may have either protective or deleterious functions, depending on the level and location (3). Its activity is regulated by endogenous inhibitors such as anti-thrombin III (serpin C1) or heparin cofactor II (serpin D1). A plasma serine protease, thrombin is synthesized in the liver as a 622 amino acid precursor with a 24 amino acid signal peptide. Cleavage by itself or by similar enzymes converts the proenzyme to three forms designated as alpha -, beta - and gamma -thrombin. Composed of a disulfide bond-linked dimer of the light chain (A) (residues 328‑363) and the heavy chain (B) (residues 364‑622), alpha -thrombin displays the diverse functions as described above. In comparison, the further processed B chains of beta - and gamma -thrombin have no known physiological function, but retain most of the activity towards small synthetic substrates (4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Coagulation Factor II/Thrombin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Goat Anti-Human Coagulation Factor II/Thrombin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1148, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). | |
Detection of Human Coagulation Factor II/Thrombin by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Coagulation Factor II/Thrombin at approximately 95 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Coagulation Factor II/Thrombin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1148) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
