Human Dkk-3 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Dkk-3 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1118-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1118-SP
别名	dickkopf (Xenopus laevis) homolog 3; dickkopf 3; dickkopf homolog 3 (Xenopus laevis); Dickkopf-3; dickkopf-related protein 3; Dkk-3; hDkk-3; regulated in glioma; REIC; REICdkk-3; RIG; RIG-like 5-6; RIG-like 7-1
全称	Dickkopf-3
反应种属	Human
应用	Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human Dkk-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse Dkk-3 is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	27122 (Human); 50781 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Loss of Dickkopf 3 Promotes the Tumorigenesis of Basal Breast Cancer PLoS ONE, 2016;11(7):e0160077. Species: Human Sample Type: Cell Lysates Application: WB Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling. Authors: Riordan D, Varma S, West R, Brown P PLoS ONE, 2015;10(7):e0128975. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded The clinicopathological significance of REIC expression in colorectal carcinomas. Authors: Wang W, Zhu W, Xu XY Histol. Histopathol., 2012;27(6):735-43. Species: Human Sample Type: Tissue Homogenates Application: WB Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development. Authors: Zhang K, Watanabe M, Kashiwakura Y, Li SA, Edamura K, Huang P, Yamaguchi K, Nasu Y, Kobayashi Y, Sakaguchi M, Ochiai K, Yamada H, Takei K, Ueki H, Huh NH, Li M, Kaku H, Na Y, Kumon H Int. J. Oncol., 2010;37(6):1495-501. Species: Human Sample Type: Whole Cells Application: ICC The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin. Authors: Choi W, Wolber R, Gerwat W J. Cell. Sci., 2010;123(0):3102-11. Species: Human Sample Type: Cell Lysates Application: WB Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB. Authors: Choi W, Miyamura Y, Wolber R, Smuda C, Reinhold W, Liu H, Kolbe L, Hearing VJ J. Invest. Dermatol., 2010;130(6):1685-96. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded The Dickkopf-homolog 3 is expressed in tumor endothelial cells and supports capillary formation. Authors: Untergasser G, Steurer M, Zimmermann M, Hermann M, Kern J, Amberger A, Gastl G, Gunsilius E Int. J. Cancer, 2008;122(7):1539-47. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Dkk-3 Ala22-Ile350 Accession # Q9UBP4
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Dkk-3, also known as REIC (Reduced Expansion in Immortalized Cells), is one of four numbered members of the Dickkopf family of Wnt antagonists (1). Dkk-3 is a secreted monomer expressed in many normal human tissues, most strongly in heart, brain and spinal cord (1, 2), and during early embryonic development in the mouse (3). N-glycosylation at up to four sites preceding or between two conserved cysteine-rich motifs results in expression of a 38 kDa glycoprotein (1, 4). The cysteine-rich motifs contain 10 cysteines each, with prokineticin and colipase families containing sequences similar to those of the second motif (1, 5). Human Dkk-3 shows 82%, 88%, 85%, and 53% amino acid (aa) identity with mouse, bovine, canine, and chick Dkk-3, respectively, and 37-45% aa identity with other human Dkk family members. Several lines of evidence implicate Dkk-3 as a negative growth regulator. Dkk-3 is downregulated in many tumors as compared to normal cells, sometimes by loss of heterozygosity (4, 6). Downregulation by CpG hypermethylation in acute lymphoblastic leukemia is correlated with faster progression and shorter survival (7). Release of cultured cells from serum starvation results in downregulation of Dkk-3 in late G1 phase of the cell cycle (6). Over-expression of Dkk‑3 results in tumor cell-line-specific growth inhibition, induction of apoptosis, and decreased tumorigenicity in nude mice (2, 4, 6). The prototype Dickkopf member, Dkk-1, antagonizes Wnt family signaling by binding to Wnt receptors LRP5 and LRP6 (low-density lipoprotein receptor-related proteins) and promoting their internalization (1, 9, 10). Results are less straightforward for Dkk-3, where some studies show binding to LRP5/6 while others do not. These effects appear to be dependent on the cells and conditions used (1, 6-10).
运输条件	Blue Ice
存放说明	4℃
参考文献	Krupnik, V.E. et al. (1999) Gene 238:301. Tsuji, T. et al. (2000) Biochem. Biophys. Res. Comm. 268:20. Kemp, C. et al. (2005) Dev. Dyn. 233:1064. Hsieh, S.-Y. et al. (2004) Oncogene 23:9183. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. Tsuji, T. et al. (2001) Biochem. Biophys. Res. Comm. 289:257. Roman-Gomez, J. et al. (2004) Br. J. Cancer 91:707. Hoang, B.H. et al. (2004) Cancer Res. 64:2734. Caricasole, A. et al. (2003) J. Biol. Chem. 278:37024. Mao, B. et al. (2001) Nature 411:321.

参考图片

Detection of Human Dkk‑3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Dkk‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1118) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Dkk‑3 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.