Human IL-19 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-19 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1035-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1035-SP
别名	IL-10C; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19
全称	Interleukin 19
反应种属	Human
应用	Western Blot(0.1 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human IL‑19 in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IL‑19-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND₅₀) is typically 1-3 µg/mL in the presence of 3 ng/mL Recombinant Human IL‑19.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	29949 (Human); 329244 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression. Authors: Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K Eur. J. Immunol., 2009;39(10):2779-88. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Potentiation of IL-19 expression in airway epithelia by IL-17A and IL-4/IL-13: important implications in asthma. Authors: Huang F, Wachi S, Thai P, Loukoianov A, Tan KH, Forteza RM, Wu R J. Allergy Clin. Immunol., 2008;121(6):1415-21, 1421. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IL-19 Leu25-Ala177 (Phe175Ser) Accession # Q9UHD0
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. Its gene contains two alternate translation initiation sites, generating precursors of 215 amino acids (aa) and 177 aa, respectively. Both isoforms are processed to 17 kDa, 153 aa mature molecules. IL-19 contains seven helices and is secreted as a 35 kDa monomer. There are two potential N-linked glycosylation sites, and it is likely that the molecule is glycosylated. Mature human IL-19 shares 69% aa sequence identity with the mature mouse homologue. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 expression is limited to activated keratinocytes and monocytes. IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (IL-20 R alpha, also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R beta or IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. Functionally, IL-19 induces IL-6 and TNF-alpha production by monocytes, and drives T-helper cell differentiation towards a Th2 response (1‑5).
运输条件	Blue Ice
存放说明	4℃
参考文献	Gallagher, G. et al. (2000) Genes Immun. 1:442. Gallagher, G. et al. (2004) Int. Immunopharmacol. 4:615. Laio, Y-C. et al. (2002) J. Immunol. 169:4288. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.

参考图片

Cell Proliferation Induced by IL‑19 and Neutralization by Human IL‑19 Antibody. Recombinant Human IL‑19 (Catalog # 1035-IL) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta in a dose‑dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑19 (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1035). The ND₅₀ is typically 1-3 µg/mL.