Human ADAM8 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human ADAM8 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1031-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1031-SP
别名	a disintegrin and metalloproteinase domain 8; ADAM 8; ADAM metallopeptidase domain 8; CD156a antigen; CD156a; CD156human leukocyte differentiation antigen; Cell surface antigen MS2; EC 3.4.24; EC 3.4.24.-; MGC134985; MS2; MS2disintegrin and metalloproteinase domain-containing protein 8
全称	A Disintegrin and Metalloprotease-like Domain 8
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunoprecipitation(25 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human ADAM8 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse ADAM8 is observed and less than 1% cross-reactivity with recombinant human (rh) ADAM12, rhADAM19, and rhADAM33 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunoprecipitation: 25 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	101 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Expression profiles and clinical correlations of degradome components in the tumor microenvironment of head and neck squamous cell carcinoma. Authors: Stokes A, Joutsa J, Ala-Aho R, Pitchers M, Pennington CJ, Martin C, Premachandra DJ, Okada Y, Peltonen J, Grenman R, James HA, Edwards DR, Kahari VM Clin. Cancer Res., 2010;16(0):2022. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM8 Ectodomain Glu158-Ser653 Accession # P78325
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	ADAM8, also known as cell surface antigen MS2 or CD156a, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). ADAM8 can cleave a variety of substrates and has been shown as a sheddase for the low affinity IgE receptor CD23 and the neural recognition molecule CHL1 (3, 4). Expression and regulation studies suggest that ADAM8 is a novel osteoclast stimulating factor and may play a role in asthma (5, 6). The 824 amino acid precursor of human ADAM8 consists of a signal peptide (residues 1 to 16), a pro peptide (residues 17 to 199), a metaloprotease domain (residues 200 to 400), a disintegrin-like domain (residues 408 to 494), a cysteine-rich region (residues 497 to 613), an EGF-like domain (residues 614 to 640), a transmembrane region (residues 656 to 676) and a cytoplasmic domain (residues 677 to 824).
运输条件	Blue Ice
存放说明	4℃
参考文献	Yoshiyama, K. et al. (1997) Genomics 41:56. Moss, M.L. and J.W. Bartsch (2004) Biochemistry 43:7227. Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469. Naus, S. et al. (2004) J. Biol. Chem. 279:16083. Choi, S.J. et al. (2001) J. Bone Miner. Res. 16:814. King, N.E. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:257.

参考图片

ADAM8 in Human PBMCs. ADAM8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human ADAM8 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1031) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.