TGF-beta 1/1.2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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TGF-beta 1/1.2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-101-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-101-SP
别名	TGFb1.2; TGF-beta 1/1.2
全称	Transforming Growth Factor beta 1 and 1.2
应用	Western Blot,Neutralization
目标/特异性	Detects TGF-beta 1/1.2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with TGF-beta 2 is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line [Tsang, M. et al. (1995) Cytokine 7:389]. The Neutralization Dose (ND₅₀) is typically 5-30 ng/mL in the presence of 1 ng/mL Recombinant Human TGF‑ beta 1 and 7.5 ng/mL Recombinant Mouse IL‑4.
来源	Polyclonal Chicken IgY
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7040 (Human); 21803 (Mouse); 59086 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment. Authors: Zhang P, Lo A, Huang Y, Huang G, Liang G, Mott J, Karpen G, Blakely E, Bissell M, Barcellos-Hoff M, Snijders A, Mao J Sci Rep, 2015;5(0):8919. Species: Mouse Sample Type: Whole Tissue Application: IHC Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta. Authors: Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K Proc Natl Acad Sci U S A, 2014;111(21):E2229-36. Species: Human Sample Type: Cell Culture Supernates Application: IP Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor beta (TGFbeta)-, epidermal growth factor (EGF)-, and nuclear factor kappaB (NFkappaB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion. Authors: Kang I, Yoon D, Braun K, Wight T J Biol Chem, 2014;289(22):15393-404. Species: Rat Sample Type: Whole Cells Application: Neut IL-21 induces IL-22 production in CD4+ T cells. Authors: Yeste A, Mascanfroni I, Nadeau M, Burns E, Tukpah A, Santiago A, Wu C, Patel B, Kumar D, Quintana F Nat Commun, 2014;5(0):3753. Species: Mouse Sample Type: Whole Cells Application: Neut The HIV-1 envelope protein gp120 impairs B cell proliferation by inducing TGF-beta1 production and FcRL4 expression. Authors: Jelicic K, Cimbro R, Nawaz F, Huang D, Zheng X, Yang J, Lempicki R, Pascuccio M, Van Ryk D, Schwing C, Hiatt J, Okwara N, Wei D, Roby G, David A, Hwang I, Kehrl J, Arthos J, Cicala C, Fauci A Nat Immunol, 2013;14(12):1256-65. Species: Human Sample Type: Whole Cells Application: Blocking Long-term gene therapy with thrombospondin 2 inhibits TGF-beta activation, inflammation and angiogenesis in chronic allograft nephropathy. Authors: Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C PLoS ONE, 2013;8(12):e83846. Species: Rat Sample Type: Whole Tissue Application: IHC Overexpression of TGF-ÃŸ 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ÃŸ. Authors: Oida T, Weiner HL J. Immunol., 2012;185(6):3529-35. Species: Human Sample Type: Cell Lysates Application: WB Homeostatic role of transforming growth factor-beta in the oral cavity and esophagus of mice and its expression by mast cells in these tissues. Authors: Vitsky A, Waire J, Pawliuk R, Bond A, Matthews D, Lacasse E, Hawes ML, Nelson C, Richards S, Piepenhagen PA, Garman RD, Andrews L, Thurberg BL, Lonning S, Ledbetter S, Ruzek MC Am. J. Pathol., 2009;174(6):2137-49. Species: Mouse Sample Type: Saliva Application: WB TGF-beta promotes thyroid epithelial cell hyperplasia and fibrosis in IFN-gamma-deficient NOD.H-2h4 mice. Authors: Yu S, Sharp GC, Braley-Mullen H J. Immunol., 2008;181(3):2238-45. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Induction of an epithelial integrin alphavbeta6 in human cytomegalovirus-infected endothelial cells leads to activation of transforming growth factor-beta1 and increased collagen production. Authors: Tabata T, Kawakatsu H, Maidji E, Sakai T, Sakai K, Fang-Hoover J, Aiba M, Sheppard D, Pereira L Am. J. Pathol., 2008;172(4):1127-40. Species: Human Sample Type: Whole Cells Application: Flow The LTK63 adjuvant improves protection conferred by Ag85B DNA-protein prime-boosting vaccination against Mycobacterium tuberculosis infection by dampening IFN-gamma response. Authors: Palma C, Iona E, Giannoni F, Pardini M, Brunori L, Fattorini L, Del Giudice G, Cassone A Vaccine, 2008;26(33):4237-43. Species: Mouse Sample Type: Whole Cells Application: Neut Characterization of in vitro antimurine thymocyte globulin-induced regulatory T cells that inhibit graft-versus-host disease in vivo. Authors: Ruzek MC, Waire JS, Hopkins D, Lacorcia G, Sullivan J, Roberts BL, Richards SM, Nahill SR, Williams JM, Scaria A, Dzuris J, Shankara S, Garman RD Blood, 2007;111(3):1726-34. Species: Mouse Sample Type: Whole Cells Application: Flow Activation of transforming growth factor-beta by the integrin alphavbeta8 delays epithelial wound closure. Authors: Neurohr C, Nishimura SL, Sheppard D Am. J. Respir. Cell Mol. Biol., 2006;35(2):252-9. Species: Human Sample Type: Whole Cells Application: Neut Tumor metastasis in an orthotopic murine model of head and neck cancer: possible role of TGF-beta 1 secreted by the tumor cells. Authors: Dasgupta S, Bhattacharya-Chatterjee M, O&amp;apos;Malley BW, Chatterjee SK J. Cell. Biochem., 2006;97(5):1036-51. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration. Authors: Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG Eur J Vasc Endovasc Surg, 2006;31(5):516-22. Species: Human Sample Type: Whole Tissue Application: IHC Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB Blood, 2005;105(2):750-8. Species: Human Sample Type: Cell Culture Supernates Application: Neut Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts. Authors: Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I Nephrol. Dial. Transplant., 2005;20(4):790-6. Species: Human Sample Type: Whole Tissue Application: IHC Neuronal TGF-beta1 mediates IL-9/mast cell interaction and exacerbates excitotoxicity in newborn mice. Authors: Mesples B, Fontaine RH, Lelievre V, Launay JM, Gressens P Neurobiol. Dis., 2005;18(1):193-205. Species: Mouse Sample Type: In Vivo Application: In vivo Autoreactive T cells persist in rats protected against experimental autoimmune encephalomyelitis and can be activated through stimulation of innate immunity. Authors: Conant SB, Swanborg RH J. Immunol., 2004;172(9):5322-8. Species: Rat Sample Type: Whole Cells Application: Neut Effect of neutralizing transforming growth factor beta1 on the immune response against Mycobacterium tuberculosis in guinea pigs. Authors: Allen SS, Cassone L, Lasco TM Infect. Immun., 2004;72(3):1358-63. Species: Guinea Pig Sample Type: In Vivo Application: In vivo Inhibition of neointimal hyperplasia after balloon injury by cis-element decoy of early growth response gene-1 in hypercholesterolemic rabbits. Authors: Ohtani K, Egashira K, Usui M, Ishibashi M, Hiasa KI, Zhao Q, Aoki M, Kaneda Y, Morishita R, Takeshita A Gene Ther., 2004;11(2):126-32. Species: Rabbit Sample Type: Whole Tissue Application: IHC Fresh
纯化方式	Antigen Affinity-purified from egg yolks
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta 1 Ala270-Ser390 Accession # P01137
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity. TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Cancer Biomarkers Cytokines and Receptors in Allergy and Asthma Early Mesodermal Lineage Markers Inflammatory Mediators Myeloid-derived Suppressor Cells (MDSC) Preeclampsia TGF-beta Family TGF-beta Family Ligands

参考图片

TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by
TGF‑ beta 1/1.2 Antibody. Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Chicken Anti-TGF‑ beta 1/1.2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-101-NA). The ND₅₀ is typically 5-30 ng/mL.