| 货号 | AF1007-SP |
| 别名 | IL-1 R9; IL-1R-9; IL1R9IL-1 receptor accessory protein-like 2; IL-1R9IL-1RAPL-2; IL-1-RAPL-2; IL1RAPL-2IL1RAPL-2-related protein; interleukin 1 receptor 9; interleukin 1 receptor accessory protein-like 2; Three immunoglobulin domain-containing IL-1 receptor-related 1; TIGIRR-1; TIGIRR-1Interleukin-1 receptor 9; X-linked interleukin-1 receptor accessory protein-like 2 | 全称 | Interleukin 1 Receptor Accessory Protein-like 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human IL‑1 RAPL2/IL‑1 R9 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse IL‑1 RAPL2/IL‑1 R9 is observed. In Western blots, approximately 5% cross‑reactivity with recombinant human (rh) IL‑1 R7 is observed and less than 1% cross-reactivity with rhIL-1 R2 and rhIL-1 R8 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 26280 (Human); 60367 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human IL‑1 RAPL2/IL‑1 R9 Thr17-Glu356 Accession # Q9NP60 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL-1 R3), ST2 (T1/IL-1 R4), IL‑18 R alpha (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL‑18 R beta (AcPL/IL-1 R7), IL-1 RAPL1 (TIGIRR-2/IL-1 R8), and IL-1 RAPL2 (TIGIRR-1/IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL‑18BP, differ from the other members by having only one Ig domain (1). IL-1 receptor accessory protein-like 2 (IL-1 RAPL2) is alternately known as IL-1 R9 and three immunoglobulin domain containing IL-1 receptor-related molecule 1 (TIGIRR-1) and is expressed in the brain (2). Its sequence predicts an 686 amino acid (aa) residue type I transmembrane glycoprotein with a 17 aa signal peptide, a 339 aa extracellular region containing three Ig-like domains, an 18 aa transmembrane domain and a 312 aa cytoplasmic tail (3). By comparison to other IL-1 receptor family proteins, IL-1 RAPL2 has a C-terminal cytoplasmic extension beyond the TIR domain that is found in IL-1 RAPL1 and SIGIRR but not other family members (3). Human and mouse IL‑1 RAPL2 share approximately 95% aa sequence identity. Human IL‑1 RAPL2 is most homologous (63%) to IL‑1 RAPL1, a receptor protein that is highly expressed in hippocampus and is involved in X-linked mental retardation (4, 5). Genes for both have been localized to human chromosome Xq22. A ligand for IL-1 RAPL2 has not been identified (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IL‑1 RAPL2/IL‑1 R9 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Goat Anti-Human IL‑1 RAPL2/IL‑1 R9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1007, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). | |
Detection of IL‑1 RAPL2/IL‑1 R9 in Hepa 1‑6 Mouse Cell Line by Flow Cytometry. Hepa 1‑6 mouse hepatoma cell line was stained with Goat Anti-Human IL‑1 RAPL2/IL‑1 R9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1007, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). |
