| 货号 | AF1003-SP |
| 别名 | AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta type II receptor; TGFbeta-RII; TGFBR2; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II | 全称 | Transforming Growth Factor beta Receptor II |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human TGF‑ beta RII Isoform 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) TGF-beta RII, recombinant mouse (rm) TGF-beta RII, rmTGF-beta RI, and rhTGF-beta RIII is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7048 (Human); 21813 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta RII Isoform 2 Thr23-Asp184 Accession # NP_001020018 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa) and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3, and appears to participate in both TGF-beta dependent and independent cellular signaling. The Type II receptor, by contrast, is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity, and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that requires the presence of the Type II receptor to bind TGF-beta. Evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors. TGF-beta receptor II isoform 2 (also TGF-beta RII isoform A) is an alternatively spliced variant of the standard Type II TGF-beta receptor (or TGF-beta RII isoform 1) that possesses a 27 amino acid substitution for Val10 near the N-terminus of the mature protein. Both TGF-beta RII and TGF-beta RII isoform 2 bind TGF-beta 1 and TGF-beta 3 with high affinity. However, only TGF-beta RII isoform 2 also binds TGF-beta 2 with high affinity in the absence of TGF-beta RIII. While TGF-beta RII is widely expressed on cells, TGF-beta RII isoform 2 shows distinct expression pattern mainly associated with bone-related cells, such as osteoblasts and mesenchymal precursor cells. TGF-beta RII isoform 2 may play an important role in TGF-beta 2 binding and signaling in cells lacking TGF-beta RIII. Mouse also expresses a TGF-beta RII isoform 2, and over aa 23-184 of this isoform, human and mouse share 80% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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