| 货号 | 9561L |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-β-Catenin (Ser33/37/Thr41) Antibody detects endogenous levels of β-catenin only when phosphorylated at serines 33, 37 or threonine 41. It does not recognize beta-catenin phosphorylated at other sites. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). |
| 存放说明 | -20C |
| 计算分子量 | 92 |
| 参考文献 | 1 . Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. 2 . Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. 3 . Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. 4 . Amit, S. et al. (2002) Genes Dev 16, 1066-76. 5 . Liu, C. et al. (2002) Cell 108, 837-47. 6 . Yanagawa, S. et al. (2002) EMBO J 21, 1733-42. 7 . Yost, C. et al. (1996) Genes Dev 10, 1443-54. 8 . Morin, P.J. et al. (1997) Science 275, 1787-90. |
Western blot analysis of extracts from 293 cells, pretreated with 20 mM LiCl for 30 minutes and then with 50 nM calyculin A, using Phospho-β-Catenin (Ser33/37/Thr41) Antibody (upper) or β-Catenin Antibody #9562 (lower). 使用Phospho-β-Catenin (Ser33/37/Thr41) 抗体 (上) 或 β-Catenin 抗体 #9562 (下)对经过 20 mM LiCl处理30分钟后和50 nM calyculin A处理的293细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from SW480 cells using Phospho-β-Catenin (Ser33/37/Thr41) Antibody. 使用Phospho-β-Catenin (Ser33/37/Thr41)抗体对SW480细胞提取物进行western blot分析。 |
