| 货号 | 9204L |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr421/Ser424. This antibody also detects p85 S6 kinase when phosphorylated at the corresponding sites (Thr444/Ser447). |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5 oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal (1). Both isoforms lie on a mitogen activated signaling pathway downstream of phosphoinositide-3 kinase (PI-3K) and the target of rapamycin, FRAP/mTOR, a pathway distinct from the Ras/MAP kinase cascade (1). The activity of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (1). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (1). Phosphorylation of Thr389, however, most closely correlates with p70 kinase activity in vivo (3). Prior phosphorylation of Thr389 is required for the action of phosphoinositide 3-dependent protein kinase 1 (PDK1) on Thr229 (4,5). Phosphorylation of this site is stimulated by growth factors such as insulin, EGF and FGF, as well as by serum and some G-protein-coupled receptor ligands, and is blocked by wortmannin, LY294002 (PI-3K inhibitor) and rapamycin (FRAP/mTOR inhibitor) (1,6,7). Ser411, Thr421 and Ser424 lie within a Ser-Pro-rich region located in the pseudosubstrate region (1). Phosphorylation at these sites is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression (1,2). Another LY294002 and rapamycin sensitive phosphorylation site, Ser371, is an in vitro substrate for mTOR and correlates well with the activity of a partially rapamycin resistant mutant p70 S6 kinase (8). |
| 存放说明 | -20C |
| 计算分子量 | 70, 85 |
Western blot analysis of extracts from 293 cells, untreated, serum-treated or EGF-treated (100 ng/ml), using Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody (upper) or p70 S6 Kinase Antibody #9202 (lower). Western blot分析293细胞提取物，分组为未处理组，血清处理组和EGF处理组(100 ng/ml)。所用抗体为Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody (上) 或p70 S6 Kinase Antibody #9202 (下)。 | |
Immunoprecipitation of phosphorylated p70 S6 Kinase from 293 cell extracts (cells were serum-stimulated as indicated) under nondenaturing or denaturing conditions, followed by Western blot analysis, using Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody. Western blot 后，免疫沉淀分析非还原和还原条件下，血清处理后的293细胞p70 S6激酶磷酸化，所用抗体为Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody。 |
