| 货号 | 3671L |
| 反应种属 | Human/Mouse/Rat/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
Western blot analysis of extracts from HEK293 cells stimulated with ionophore A23187 for the indicated times, using Phospho-Myosin Light Chain 2 (Ser19) Antibody. 使用Phospho-Myosin Light Chain 2 (Ser19) Antibody，免疫印迹（Western Blot）分析HEK293细胞中Phospho-Myosin Light Chain 2 (Ser19)蛋白水平，细胞如图时间所示给予ionophore A23187处理。 | |
Confocal immunofluorescent images of HeLa cells, untreated (left) or phosphatase-treated (right), labeled with Phospho-Myosin Light Chain 2 (Ser19) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Phospho-Myosin Light Chain 2 (Ser19) Antibody (绿色)标记，共聚焦免疫荧光分析HeLa细胞，细胞分为untreated (左图)或phosphatase-treated (右图)。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。蓝色伪彩= DRAQ5® #4084（DNA荧光染料）。 |
