| 货号 | 2401L |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IHC-F/IF-IC |
| 目标/特异性 | Phospho-HSP27 (Ser82) Antibody detects endogenous HSP27 only when phosphorylated at serine 82. The antibody does not recognize other heat shock proteins. |
| 使用方法 | WB(1:1000) IHC-P (1:50) IF-IC (1:25) IHC-F (1:50) |
| 供应商 | CST |
| 背景 | Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78 and Ser82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 27 |
Western blot analysis of extracts from HeLa cells, using Phospho-HSP27 (Ser82) Antibody (upper) or control HSP27 antibody (lower). HeLa cells were incubated at 42ºC for 0-2 hours as indicated. 对HeLa细胞抽提液，使用Phospho-HSP27 (Ser82) Antibody（上图）或对照HSP27 antibody（下图）进行Western blot分析。Hela细胞按要求在42℃中孵育0-2小时。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (A,C) or lambda phosphatase-treated (B,D), using Phospho-HSP27 (Ser82) Antibody (A,B) or HSP27 (G31) Monoclonal Antibody #2402 (C,D). 对石蜡包埋的人乳腺癌，对照（A,C）或用γ磷酸酶处理（B,D），使用Phospho-HSP27 (Ser82) Antibody (A,B)或HSP27 (G31) Monoclonal Antibody #2402 (C,D)进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-HSP-27 (Ser82) Antibody in the presence of control peptide (left) or antigen specific peptide (right). 对石蜡包埋的人肺癌使用Phospho-HSP-27 (Ser82) Antibody进行免疫组织化学分析，其中左图为对照肽，右图为抗原特异肽。 | |
Immunohistochemical analysis of frozen H1650 xenograft, showing cytoplasmic localization using Phospho-HSP27 (Ser82) Rabbit mAb. 对冰冻H1650异种移植物使用Phospho-HSP27 (Ser82) Rabbit mAb兔单抗进行免疫组化分析，显示出胞质定位。 | |
Confocal immunofluorescent analysis of HeLa cells, anisomycin-treated (left) or untreated (right), using Phospho-HSP27 (Ser82) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对HeLa细胞，加入anisomycin处理（左）或未处理（右），使用Phospho-HSP27 (Ser82) Antibody（绿色）进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor®555 phalloidin（红色）标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Western blot analysis of extracts of various cell lines, using Phospho-HSP27 (Ser82) Antibody #2401 (upper) or control HSP27 Antibody #2402 (lower). 对多个细胞抽提液，使用Phospho-HSP27 (Ser82) Antibody #2401（上图）或对照HSP27 Antibody #2402 （下图）进行Western blot分析。 |
