| 货号 | 2234L |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | Phospho-EGF Receptor (Tyr1068) Antibody detects endogenous levels of EGF receptor only when phosphorylated at tyrosine 1068. The antibody may cross-react with other activated EGF receptor family members (e.g. ErbB2), and cross-reacts slightly with activated PDGF receptor . |
| 使用方法 | WB(1:1000) IHC-P (1:350) |
| 供应商 | CST |
| 背景 | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provides a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). |
| 存放说明 | -20C |
| 计算分子量 | 175 |
Immunohistochemical analysis of paraffin-embedded human breast tumor, showing membrane and cytoplasmic localization, using Phospho-EGF Receptor (Tyr1068) Antibody.用抗磷酸化EGF受体(Tyr1068) 的抗体对石蜡包埋的人乳腺肿瘤组织进行免疫印组化检测，可见包膜和胞浆着色。 | |
Immunohistochemical annalysis of paraffin-embedded MDA-MB-468 cells, untreated (left), EGF treated (middle), or EGF and Tarceva® treated (right), using Phospho-EGF Receptor (Tyr1068) Antibody.用抗磷酸化EGF受体(Tyr1068) 的抗体对石蜡包埋的下列细胞进行免疫印组化检测： 未经处理的（中），经（左）EGF 处理的，或经EGF和Tarceva®共同处理的（右）MDA-MB-468细胞。 | |
Western blot analysis of MDA-468 cells untreated and treated with EGF, using Phospho-EGF Receptor (Tyr1068) Antibody.用抗磷酸化EGF受体(Tyr1068) 的抗体对经过或未经EGF处理的A431 细胞的裂解液进行免疫印迹检测。 |
