Phospho-4E-BP1 (Thr37/46) Antibody
货号：
9459L 基本售价：
9300.0 元 规格：
-
产品信息
概述| 货号 | 9459L |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-4E-BP1 (Thr37/46) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). |
| 存放说明 | -20C |
| 计算分子量 | 15 to 20 |
参考图片Western blot analysis of extracts from 293T cells using 4E-BP1 Antibody #9452 (upper) and Phospho-4E-BP1 (Thr37/46) Antibody (lower). The cells were starved for 24 hours in serum-free medium and underwent a 1 hour amino acid deprivation. Amino acids were replenished for 1 hour. Cells were then either untreated (-) or treated with 100 nM insulin (+) for 30 minutes. 使用4E-BP1 Antibody #9452 (上图)和Phospho-4E-BP1 (Thr37/46) Antibody (下图)，免疫印迹（Western Blot）分析在293 T细胞系裂解物。细胞在无血清饥饿培养24小时，并氨基酸缺乏处理1小时，然后再重新加入氨基酸处理1小时。然后细胞再加入100 nM胰岛素(+)或不加胰岛素处理(-)30分钟。 |
