| 货号 | 9621L |
| 反应种属 | Human/Mouse/Rat/All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/E-P |
| 目标/特异性 | Phospho-(Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine residue with arginine at the -3 position. It is a useful tool in identifying substrates of AGC family kinases, including PKA and PKC. It does not cross-react with the nonphosphorylated PKA substrate motif. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:200) E-P (1:1000) |
| 供应商 | CST |
| 背景 | An important class of kinases, refered to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery. |
| 存放说明 | -20C |
Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser/Thr) PKA Substrate Antibody. 对未处理或calyculin A处理的A431细胞抽提液使用Phospho-(Ser/Thr) PKA Substrate Antibody进行Western blot分析。 | |
Western blot analysis of extracts from A431 cells, phosphorylated in vitro by protein kinase A, Erk2 or cdc2/cyclin A, plus or minus PKA inhibitor (PKI), using Phospho-(Ser/Thr) PKA Substrate Antibody. 对体外蛋白激酶A、Erk2或cdc2/cyclin A磷酸化，加或不加PKA抑制剂（PKI）的A431细胞，使用Phospho-(Ser/Thr) PKA Substrate Antibody进行Western blot分析。 | |
Phospho-(Ser/Thr) PKA Substrate Antibody ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.) Phospho-(Ser/Thr) PKA Substrate Antibody ELISA分析：磷酸化和非磷酸化的信噪比。（T*和S*代表磷酸化的苏氨酸和丝氨酸） | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phsopho-(Ser/Thr) PKA Substrate Antibody. 对石蜡包埋的人乳腺癌对照（左）和λ磷酸酶处理（右），使用Phsopho-(Ser/Thr) PKA Substrate Antibody进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-(Ser/Thr) PKA Substrate Antibody. 对石蜡包埋的人肺癌使用Phospho-(Ser/Thr) PKA Substrate Antibody进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-(Ser/Thr) PKA Substrate Antibody. 对石蜡包埋的人结肠癌使用Phospho-(Ser/Thr) PKA Substrate Antibody进行免疫组化分析。 |
