| 货号 | 2851L |
| 反应种属 | Human/Monkey/All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/E-P |
| 目标/特异性 | Phospho-(Ser/Thr) ATM/ATR Substrate Antibody detects endogenous levels of proteins containing the ATM/ATR substrate motif. This antibody preferentially binds peptides and proteins that contain phospho-Ser/Thr preceded by Leu or similar hydrophobic amino acids at the -1 position and followed by Gln at the +1 position. The antibody does not cross-react with corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) IP (1:20) IHC-P (1:100) E-P (1:1000) |
| 供应商 | CST |
| 背景 | Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair (1). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1 (1,2) The essential requirement for the substrates of ATM/ATR is S*/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation (3). The complex phenotype of AT cells suggests that it likely has additional substrates (3). To better understand the kinase and identify substrates for ATM and the related kinase ATR, CST has developed antibodies that recognize phosphorylated serine or threonine in the S*/T*Q motif. |
| 存放说明 | -20C |
Western blot analysis of extracts from COS cells, untreated or UV-treated, using Phospho-(Ser/Thr) ATM/ATR Substrate Antibody. 对未处理或UV处理的COS细胞抽提液使用Phospho-(Ser/Thr) ATM/ATR Substrate Antibody进行Western blot分析。 | |
Western blot analysis of yeast cell extracts, untreated or treated with 4-NQO, a potent activator of ATM/ATR orthologues, using Phospho-(Ser/Thr) ATM/ATR Substrate Antibody. 对HeLa细胞抽提液，未处理或4-NQO（强力ATM/ATR同源物激活剂）处理，使用Phospho-(Ser/Thr) ATM/ATR Substrate Antibody进行Western blot分析。 | |
Western blot analysis of immunoprecipitated protein from Chk2-transfected and UV-treated COS cells, using Chk2 antibody for immunoprecipitation and Phospho-(Ser/Thr) ATM/ATR Substrate Antibody for immunoblotting. 对转染Chk2和UV处理的COS细胞，使用Chk2 antibody进行免疫共沉淀，使用Phospho-(Ser/Thr) ATM/ATR Substrate Antibody进行免疫印迹。 | |
Phospho-(Ser/Thr) ATM/ATR Substrate Antibody ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (S* or T* denote phosphorylated serine or threonine.) Phospho-(Ser/Thr) ATM/ATR Substrate Antibody ELISA分析：磷酸化和非磷酸化的信噪比。（S*或T*代表磷酸化的丝氨酸或苏氨酸） |
