| 货号 | 9611L |
| 反应种属 | Human/Mouse/Rat/All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/E-P |
| 目标/特异性 | Phospho-(Ser/Thr) Akt Substrate Antibody preferentially recognizes peptides and proteins containing phospho-Ser/Thr preceded by Lys/Arg at positions -5 and -3, in a manner largely independent of other surrounding amino acids. Some cross-reactivity is observed for peptides that contain phospho-Ser/Thr preceded by Arg/Lys at positions -3 and -2. No cross-reactivity is observed with the corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs. By ELISA, the antibody recognizes a wide range of phosphorylated Akt substrate peptides, and, by 2-D gel Western blot analysis, it recognizes a large number of proteins presumed to be Akt substrates. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:500) E-P (1:500) |
| 供应商 | CST |
| 背景 | An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from CST are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. |
| 存放说明 | -20C |
Western blot analysis of extracts from Jurkat cells, untreated or calyculin A-treated (0.1 µM for 30 minutes prior to lysis), using Phospho-(Ser/Thr) Akt Substrate Antibody. Proteins were separated by 2-D electrophoresis prior to blotting. 对未处理或0.1uM calyculin A裂解前处理30分钟的Jurkat细胞抽提液使用Phospho-(Ser/Thr) Akt Substrate Antibody进行Western blot分析。蛋白质在印记前在二维电泳分离。 | |
Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser/Thr) Akt Substrate Antibody. 对未处理或calyculin A处理的A431细胞抽提液使用Phospho-(Ser/Thr) Akt Substrate Antibody进行Western blot分析。 | |
Phospho-(Ser/Thr) Akt Substrate Antibody ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.) Phospho-(Ser/Thr) Akt Substrate Antibody ELISA分析：磷酸化和非磷酸化的信噪比。（T*和S*代表磷酸化的苏氨酸和丝氨酸） | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-(Ser/Thr) Akt Substrate Antibody. 对石蜡包埋的人肺癌使用Phospho-(Ser/Thr) Akt Substrate Antibody进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-(Ser/Thr) Akt Substrate Antibody. 对石蜡包埋的人结肠癌使用Phospho-(Ser/Thr) Akt Substrate Antibody进行免疫组化分析。 | |
Western blot analysis of extracts from C2C12 cells, untreated or insulin-treated (100 nM for 15 minutes prior to lysis), using Phospho-(Ser/Thr) Akt Substrate Antibody. 对未处理或100 nM 胰岛素裂解前处理15分钟的Jurkat细胞抽提液使用Phospho-(Ser/Thr) Akt Substrate Antibody进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phospho-(Ser/Thr) Akt Substrate Antibody. 对石蜡包埋的人乳腺癌对照（左）和λ磷酸酶处理（右），使用Phospho-(Ser/Thr) Akt Substrate Antibody进行免疫组化分析。 |
