| 货号 | 9742L |
| 反应种属 | Human/Mouse/Rat/Monkey/Mink/Zebrafish |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IHC-F |
| 目标/特异性 | eIF4E Antibody detects endogenous levels of total eIF4E protein. The antibody does not cross-react with other proteins. |
| 使用方法 | WB(1:1000) IHC-P (1:100) IHC-F (1:100) |
| 供应商 | CST |
| 背景 | Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin. |
| 存放说明 | -20C |
| 计算分子量 | 25 |
Western blot analysis of extracts from control and serum treated NIH/3T3, 293 or PC12 cells, using eIF4E Antibody. 使用eIF4E Antibody，免疫印迹（Western Blotting）分析对照组和血清处理过的NIH/3T3, 293和PC12细胞系提取物。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic localization, using eIF4E Antibody. 使用eIF4E Antibody，免疫分析人类肺癌组织石蜡切片，结果显示eIF4E蛋白定位于细胞质。 | |
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using eIF4E Antibody. 使用eIF4E Antibody，免疫组化分析人类肾脏细胞癌石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human colon, using eIF4E Antibody in the presence of control peptide (left) or antigen-specific peptide (right). 使用eIF4E Antibody，免疫组化分析人类结肠组织石蜡切片，其中切片分别添加对照多肽 (左图)和抗原特异性多肽 (右图)。 | |
Immunohistochemical analysis of frozen H1650 xenograft, using eIF4E Antibody. 使用eIF4E Antibody，免疫组化分析H1650异种移植物的冰冻切片。 |
