| 货号 | 6949S |
| 描述 | This Cell Signaling Technology® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381. |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/E-P |
| 目标/特异性 | Phospho-Threonine Antibody (P-Thr-Polyclonal) (HRP Conjugate) detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. The antibody is phospho-specific and may cross-react with some phospho-serine-containing sequences. By ELISA, it recognizes a wide variety of threonine-phosphorylated peptides, and by 2D gel western blot analysis, it recognizes a large number of presumably threonine-phosphorylated proteins. We recommend the use of Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) #9391 to detect proteins containing threonine followed by proline. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) E-P (1:2000) |
| 供应商 | CST |
| 标记 | HRP |
| 背景 | Much of the dynamic behavior of cellular proteins, including the regulation of molecular interactions (1), subcellular localization (2), and transcriptional regulation (3) is controlled by a variety of post-translational modifications (4). Antibodies specific for these post-translational modifications are invaluable tools in the quest to understand normal and pathogenic molecular and cellular behavior. General protein modification antibodies are designed to react with modified amino acid residues (e.g., phospho-threonine, phospho-tyrosine, acetyl-lysine, nitro-tyrosine) independently of the sequence in which they are embedded. This ability to recognize modified residues in a "context independent" fashion gives these antibodies broad reactivities, presumably conferring upon them the ability to react with hundreds of distinct proteins. This broad pattern of reactivity makes these antibodies especially valuable in multiplex analyses and target discovery programs. |
| 存放说明 | -20C |
Western blot analysis of extracts from Jurkat cells, untreated or treated with Calyculin A #9902 (0.1 µM for 45 minutes), using Phospho-Threonine Antibody (P-Thr-Polyclonal) (HRP Conjugate). 对未处理或0.1uM calyculin A裂解前处理45分钟的Jurkat细胞抽提液使用Phospho-Threonine Antibody (P-Thr-Polyclonal) (HRP Conjugate)进行Western blot分析。 | |
Signal-to-noise (S/N) values of phosphopeptide versus nonphosphopeptide signals using LumiGLO® and Peroxide Reagents #7003. 使用LumiGLO® and Peroxide Reagents #7003的磷酸化肽段和非磷酸化肽段的信噪比。 |
