| 货号 | 58014S |
| 反应种属 | H |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-ATR (Thr1989) Antibody recognizes endogenous levels of ATR protein only when phosphorylated at Thr1989. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). Despite the essential role of ATR in cell cycle signaling and DNA repair processes, little is known about its activation. ATR was long thought to exist in a constitutively active state in cells, with DNA damage-induced signaling occurring via recruitment of ATR to single stranded DNA and sites of replication stress. Phosphorylation of ATR at serine 428 in response to UV-induced DNA damage has been suggested as a means of activating ATR (4,5). Recent work has shown autophosphorylation of ATR at threonine 1989. Like ATM Ser1981, phosphorylation of ATR Thr1989 occurs in response to DNA damage, indicating that phosphorylation at this site is important in ATR-mediated signaling (6,7). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 300 |
| 参考文献 | 1 . Kastan, M.B. and Lim, D.S. (2000) Nat Rev Mol Cell Biol 1, 179-86. 2 . Abraham, R.T. (2004) DNA Repair (Amst) 3, 883-7. 3 . Shechter, D. et al. (2004) DNA Repair (Amst) 3, 901-8. 4 . Vauzour, D. et al. (2007) Arch Biochem Biophys 468, 159-66. 5 . Smith, J. et al. (2010) Adv Cancer Res 108, 73-112. 6 . Nam, E.A. et al. (2011) J Biol Chem 286, 28707-14. 7 . Liu, S. et al. (2011) Mol Cell 43, 192-202. |
Western blot analysis of extracts from 293T cells transfected with an expression plasmid encoding a Myc/DDK-tagged fragment of either WT or T1989A mutant human ATR. Western blot was performed using Phospho-ATR (Thr1989) Antibody (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower). | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Mitomycin C (MMC) (2 μg/mL, 24 hr; +). ATR inhibitor VE-821 (10 μM; +) and/or ATM inhibitor KU55933 (10 μM, +) were added 1 hour prior to MMC treatment. Western blot was performed using Phospho-ATR (Thr1989) Antibody (upper) or ATR (E1S3S) Rabbit mAb #13934 (lower). |
