| 货号 | 52718S |
| 反应种属 | M |
| 来源宿主 | Rabbit |
| 应用 | W IP |
| 目标/特异性 | Cleaved-IL-1β (Asp117) Antibody (Mouse Specific) recognizes endogenous levels of mouse IL-1β protein only when cleaved at Asp117. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity. |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 17 |
| 参考文献 | 1 . Dinarello, C.A. (1998) Int Rev Immunol 16, 457-99. 2 . Burns, K. et al. (1998) J Biol Chem 273, 12203-9. 3 . Cao, Z. et al. (1996) Nature 383, 443-6. 4 . Cao, Z. et al. (1996) Science 271, 1128-31. 5 . Wesche, H. et al. (1997) Immunity 7, 837-47. 6 . Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6. 7 . Thornberry, N.A. et al. (1992) Nature 356, 768-74. 8 . Cerretti, D.P. et al. (1992) Science 256, 97-100. |
Western blot analysis of recombinant Mouse Interleukin-1β (mIL-1β) #5204 using Cleaved-IL-1β (Asp117) Antibody (Mouse Specific). | |
Immunoprecipitation of mouse cleaved-IL-1β (Asp117) from extracts of C2C12 cells spiked with recombinant Interleukin-1β (mIL-1β) #5204 (1ng/ml). Lane 1 is 10% input, lane 2 is precipitated with Normal Rabbit IgG #2729 and lane 3 is precipitated with Cleaved-IL-1β (Asp117) Antibody (Mouse Specific). Western blot was performed using Cleaved-IL-1β (Asp117) Antibody (Mouse Specific). | |
Western blot analysis of cell extracts and media from mouse bone marrow derived macrophages (mBMDM), untreated (-) or treated with Lipopolysaccharides #14011 (LPS; 50ng/ml, 4hr) followed by nigericin (15μM, 45min) (+) using Cleaved-IL-1β (Asp117) Antibody (Mouse Specific) (upper), or total IL-1β (D3H1Z) Rabbit mAb (Mouse Specific) #12507 (lower). |
