| 货号 | 29957S |
| 反应种属 | Human\Mouse\Rat |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Phospho-Tau (Ser199) Antibody |
| 使用方法 | W |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimers disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3). |
| 存放说明 | -20C |
| 计算分子量 | 50-80 |
| 参考文献 | 1 . Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9. 2 . Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76. 3 . Bramblett, G.T. et al. (1993) Neuron 10, 1089-99. 4 . Qian, W. et al. (2010) J Alzheimers Dis 19, 1221-9. 5 . Leroy, A. et al. (2010) J Biol Chem 285, 33435-44. 6 . Thornton, C. et al. (2011) Biochem J 434, 503-12. 7 . Jin, N. et al. (2015) J Biol Chem 290, 15219-37. 8 . Mondragón-Rodríguez, S. et al. (2008) Neuropathol Appl Neurobiol 34, 62-75. 9 . Mondragón-Rodríguez, S. et al. (2008) Int J Exp Pathol 89, 81-90. |
Western blot analysis of extracts from mouse and rat brain using Phospho-Tau (Ser199) Antibody. The phospho-specificity of Phospho-Tau (Ser199) Antibody was verified by peptide blocking using a phosphopeptide or non-phosphopeptide targeting residue Ser199. |
