| 货号 | 9763S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | Tri-Methyl-Histone H3 (Lys36) Antibody detects endogenous levels of histone H3 only when tri-methylated on Lys36. The antibody does not cross-react with non-methylated, mono-methylated, or di-methylated Lys36. In addition, the antibody does not cross-react with methylated histone H3 Lys4, Lys9, Lys27 or methylated histone H4 Lys20. |
| 使用方法 | WB(1:1000) IHC-P (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9). |
| 存放说明 | -20C |
| 计算分子量 | 17 |
| 参考文献 | 1 . Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51. 2 . Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27. 3 . Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42. 4 . Lee, D.Y. et al. (2005) Endocr Rev 26, 147-70. 5 . Daniel, J.A. et al. (2005) Cell Cycle 4, 919-26. 6 . Shi, X. et al. (2006) Nature 442, 96-9. 7 . Wysocka, J. et al. (2006) Nature 442, 86-90. 8 . Wysocka, J. et al. (2005) Cell 121, 859-72. 9 . Trojer, P. and Reinberg, D. (2006) Cell 125, 213-7. |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Tri-Methyl-Histone H3 (Lys36) Antibody. 使用Tri-Methyl-Histone H3 (Lys36) Antibody，免疫组化分析人源乳腺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Tri-Methyl-Histone H3 (Lys36) Antibody in the presence of control peptide (left) or antigen specific peptide (right). 使用Tri-Methyl-Histone H3 (Lys36) Antibody，左图：control peptide，右图：antigen specific peptide，免疫组化分析人源结肠腺癌组织石蜡切片。 | |
Confocal immunofluorescent analysis of HeLa cells using Tri-Methyl-Histone H3 (Lys36) Antibody (green). Actin filaments have been labeled with DY-554 (red). 使用Tri-Methyl-Histone H3 (Lys36) Antibody (绿色)，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of extracts from various cell lines using Tri-Methyl-Histone H3 (Lys36) Antibody. 使用Tri-Methyl-Histone H3 (Lys36) Antibody，免疫印迹(Western blot)分析不同细胞中Tri-Methyl-Histone H3 (Lys36)的蛋白水平。 | |
Tri-Methyl-Histone H3 (Lys36) Antibody specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated tri-methyl histone H3 (Lys36) peptide in the presence of increasing concentrations of various competitor peptides. As shown, only the tri-methyl histone H3 (Lys36) peptide competed away binding of the antibody. 通过peptide ELISA确定Tri-Methyl-Histone H3 (Lys36) Antibody的特异性。该图描述了抗体与提前包被的tri-methyl histone H3 (Lys36) peptide的结合能力，并且多肽中含有浓度递增的不同竞争多肽。如同所示，仅tri-methyl histone H3 (Lys36) peptide竞争脱离抗体的结合。 |
