| 货号 | 9567S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-β-Catenin (Ser675) Antibody detects endogenous levels of β-catenin only when phosphorylated at Ser675. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). |
| 存放说明 | -20C |
| 计算分子量 | 92 |
| 参考文献 | 1 . Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. 2 . Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. 3 . Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. 4 . Amit, S. et al. (2002) Genes Dev 16, 1066-76. 5 . Liu, C. et al. (2002) Cell 108, 837-47. 6 . Yanagawa, S. et al. (2002) EMBO J 21, 1733-42. 7 . Yost, C. et al. (1996) Genes Dev 10, 1443-54. 8 . Morin, P.J. et al. (1997) Science 275, 1787-90. 9 . Hino S et al. (2005) Mol Cell Biol 25, 9063–72 10 . Taurin, S. et al. (2006) J. Biol. Chem. 281, 9971-9976. |
COS-7 cells were transfected with cDNA encoding DYKDDDDK-tagged wild type (WT) β-catenin or Ser-to-Ala β-catenin mutants as indicated. Cells were stimulated with 10 mM forskolin (FSK) for 5 minutes and lysed. β-catenin or its mutants were immunoprecipitated with DYKDDDDK Tag Antibody (Binds to same epitope as Sigmas Anti-FLAG® M2 Antibody) #2368 and analyzed by western blotting using Phospho-β-Catenin (Ser552) Antibody #9566, Phospho-β-Catenin (Ser675) Antibody or DYKDDDDK Tag Antibody as indicated (Figures provided by Drs. Sebastien Taurin and Nickolai Dulin, Department of Medicine/Pulmonary, University of Chicago). DYKDDDDK标记的野生型（WT）β-catenin或Ser-to-Ala β-catenin突变体cDNA转染COS-7细胞后如图所示。10 mM forskolin (FSK)处理细胞5分钟后，溶解的β-catenin 或其突变体用DYKDDDDK Tag抗体免疫沉淀（结合到same epitope as Sigmas Anti-FLAG® M2抗体）#2368并使用Phospho-β-Catenin (Ser552)抗体 #9566, Phospho-β-Catenin (Ser675) 抗体#9567, 或 DYKDDDDK Tag抗体进行western blot实验。（图片由芝加哥大学Department of Medicine / Pulmonary, Sebastien Taurin和 Nickolai Dulin博士提供） | |
Western blot analysis of extracts from SK-N-MC cells, treated with forskolin (FSK) for 30 minutes or λ phosphatase for 1 hour, using Phospho-β-Catenin (Ser675) Antibody (upper) or β-Catenin Antibody (Amino-terminal Antigen) #9581 (lower). 使用hospho-β-Catenin (Ser675) 抗体（上）或 β-Catenin 抗体（氨基端抗原）#9581（下）对经过forskolin (FSK) 处理30分钟或λ磷酸酶处理1小时的SK-N-MC细胞进行western blot分析。 |
