Phospho-β-Catenin (Ser45) Antibody【科瑞奥博】

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Phospho-β-Catenin (Ser45) Antibody

货号： 9564S 基本售价： 3980.0 元规格： -

产品信息

概述
货号	9564S
反应种属	Human
来源宿主	Rabbit
应用	W
目标/特异性	Phospho-β-Catenin (Ser45) Antibody detects endogenous levels of beta-catenin only when phosphorylated at serine 45. It does not recognize β-catenin phosphorylated at other sites.
使用方法	WB(1:1000)

性能
供应商	CST
灵敏度	Endogenous
背景	β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
存放说明	-20C
计算分子量	92
参考文献	1 . Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. 2 . Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. 3 . Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. 4 . Amit, S. et al. (2002) Genes Dev 16, 1066-76. 5 . Liu, C. et al. (2002) Cell 108, 837-47. 6 . Yanagawa, S. et al. (2002) EMBO J 21, 1733-42. 7 . Yost, C. et al. (1996) Genes Dev 10, 1443-54. 8 . Morin, P.J. et al. (1997) Science 275, 1787-90.

参考图片

Western blot analysis of extracts from HEK293 cells untreated and treated with 50 nM calyculin A for 30 minutes, using Phospho-β-Catenin (Ser45) Antibody (lanes 1-2) or the same antibody after preincubation with listed peptides (lanes 3-8). (S45-P is phospho-Ser45 peptide; S33/37/T41-P is phospho-Ser33/37/Thr41 peptide; S45-non-P is nonphospho-Ser45 peptide.)

使用Phospho-β-Catenin (Ser45) 抗体（1-2道）或与图示中的多肽提前孵育过的抗体（3-8道），对未处理或经过50 nM calyculin A处理30分钟的293细胞提取物进行western blot分析。(S45-P是 phospho-Ser45 peptide; S33/37/T41-P 是phospho-Ser33/37/Thr41 peptide; S45-non-P是nonphospho-Ser45 peptide.)

Western blot analysis of extracts from HEK293 cells untreated and treated with calyculin A (50 nM for 30 minutes), using Phospho-β-Catenin (upper) and β-Catenin Antibody #9562 (lower).

使用Phospho-β-Catenin (上)和 β-Catenin 抗体#9562 (下)对未处理和经过calyculin A (50 nM 30分钟)的HEK293细胞提取物进行western blot分析。

Western blot analysis of extracts from SW480 cells, using Phospho-β-Catenin (Ser45) Antibody.

使用Phospho-β-Catenin (Ser45)抗体对SW480细胞提取物进行western blot分析。