| 货号 | 2009S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-β-Catenin (Ser33/37) Antibody detects endogenous levels of β-catenin only when phosphorylated at Ser33 and Ser37. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). |
| 存放说明 | -20C |
| 计算分子量 | 92 |
| 参考文献 | 1 . Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. 2 . Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. 3 . Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. 4 . Amit, S. et al. (2002) Genes Dev 16, 1066-76. 5 . Liu, C. et al. (2002) Cell 108, 837-47. 6 . Yanagawa, S. et al. (2002) EMBO J 21, 1733-42. 7 . Yost, C. et al. (1996) Genes Dev 10, 1443-54. 8 . Morin, P.J. et al. (1997) Science 275, 1787-90. |
Western blot analysis of extracts from SW620 cells, untreated or treated with Calyculin A #9902, using Phospho-β-Catenin (Ser33/37) Antibody. 使用Phospho-β-Catenin (Ser33/37)抗体对未处理或Calyculin A#9902处理的SW620细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from SW620 cells, untreated or treated with λ phosphatase, using Phospho-β-Catenin (Ser33/37) Antibody. |
