| 货号 | 13231S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-α-E-Catenin (Ser655/Thr658) Antibody recognizes endogenous levels of α-E-catenin protein only when phosphorylated at Ser655 and Thr658. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Research studies have demonstrated that loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Kobielak, A. and Fuchs, E. (2004) Nat Rev Mol Cell Biol 5, 614-25. 2 . Yamada, S. et al. (2005) Cell 123, 889-901. 3 . Drees, F. et al. (2005) Cell 123, 903-15. 4 . Hwang, S.G. et al. (2005) J Biol Chem 280, 12758-65. 5 . Rigbolt, K.T. et al. (2011) Sci Signal 4, rs3. 6 . Brill, L.M. et al. (2009) Cell Stem Cell 5, 204-13. 7 . Huttlin, E.L. et al. (2010) Cell 143, 1174-89. 8 . Pan, C. et al. (2008) Proteomics 8, 4534-46. |
Western blot analysis of extracts from NCI-H3255 cells, untreated (-) or λ phosphatase-treated (+), using Phospho-α-E-Catenin (Ser655/Thr658) Antibody (upper), α-E-Catenin (23B2) Rabbit mAb #3240 (middle) and β-Actin (D6A8) Rabbit mAb #8457 (lower).未处理(-)或λ phosphatase处理(+)的NCI-H3255细胞，使用Phospho-α-E-Catenin (Ser655/Thr658) Antibody (上), α-E-Catenin (23B2) Rabbit mAb #3240 (中) 和 β-Actin (D6A8) Rabbit mAb #8457 (下)进行western blot分析。 | |
Immunoprecipitation of phospho-α-E-catenin protein from PANC-1 cell extracts using Normal Rabbit IgG #2729 (lane 2) or Phospho-α-E-Catenin (Ser655/Thr658) Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-α-E-Catenin (Ser655/Thr658) Antibody. 使用Normal Rabbit IgG #2729 (lane 2) 或Phospho-α-E-Catenin (Ser655/Thr658) Antibody (lane 3)对PANC-1细胞提取物中的phospho-α-E-catenin蛋白进行免疫沉淀实验。Lane 1是10%上样量。使用Phospho-α-E-Catenin (Ser655/Thr658) Antibody进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using Phospho-α-E-Catenin (Ser655/Thr658) Antibody.使用Phospho-α-E-Catenin (Ser655/Thr658) Antibody对多种细胞提取物进行western blot分析。 | |
Phospho-α-E-Catenin (Ser655/Thr658) Antibody specificity was determined by peptide competition ELISA. The binding of Phospho-α-E-Catenin (Ser655/Thr658) Antibody to phospho-α-E-catenin (Ser655/Thr658) peptide in the presence of increasing concentrations of competitor peptides is shown. Only the di-phospho-α-E-catenin (Ser655/Thr658) peptide competes for binding. Phospho-α-E-Catenin (Ser655/Thr658) Antibody特异性由肽竞争ELISA确认。浓度逐渐增加的竞争肽存在条件下Phospho-α-E-Catenin (Ser655/Thr658) Antibody和phospho-α-E-catenin (Ser655/Thr658) peptide的结合如图所示。仅有di-phospho-α-E-catenin (Ser655/Thr658)能竞争结合。 |
