| 货号 | 4911S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-YAP (Ser127) Antibody detects endogenous levels of YAP only when phosphorylated at serine 127. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). Upon phosphorylation at Ser127, YAP interacts with 14-3-3 in an Akt-dependent manner and suppresses p73-mediated apoptosis (6). |
| 存放说明 | -20C |
| 计算分子量 | 65 to 75 |
| 参考文献 | 1 . Sudol, M. (1994) Oncogene 9, 2145-52. 2 . Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90. 3 . Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23. 4 . Sudol, M. et al. (1995) FEBS Lett 369, 67-71. 5 . Yagi, R. et al. (1999) EMBO J 18, 2551-62. 6 . Basu, S. et al. (2003) Mol Cell 11, 11-23. 7 . Zhao, B. et al. (2010) Genes Dev 24, 862-74. 8 . Zhao, B. et al. (2007) Genes Dev 21, 2747-61. 9 . Yu, F.X. et al. (2012) Cell 150, 780-91. 10 . Zhao, B. et al. (2010) Genes Dev 24, 72-85. |
Western blot analysis of extracts from MDA-MB-468 cells, untreated or treated with wortmannin and LY294002, using Phospho-YAP (Ser127) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after western transfer.使用Phospho-YAP (Ser127)对未处理或经过渥曼青霉素和LY294002处理后的MDA-MB-468 细胞提取物进行Western blot分析。本抗体的磷酸化特异性验证通过小肠磷酸酶（CIP）处理或不处理western转膜结束后的膜进行。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic and nuclear localization, using Phospho-YAP (Ser127) Antibody.使用小肠磷酸酶（CIP）处理或不处理对石蜡包埋的人结肠癌组织进行免疫组化，显示细胞质和细胞核定位。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-YAP (Ser127) Antibody in the presence of control peptide (left) or antigen specific peptide (right).在对照多肽（左）和抗原特异性多肽（右）存在的条件下使用Phospho-YAP (Ser127) 对石蜡包埋的人乳腺癌进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma control (left) or lambda phosphatase-treated (right), using Phospho-YAP (Ser127) Antibody使用Phospho-YAP (Ser127) 抗体对对照组（左）或lambda磷酸酶处理后（右）石蜡包埋的人肺癌组织进行免疫组化分析。 |
