| 货号 | 4946S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-WNK1 (Thr60) Antibody detects endogenous levels of WNK1 only when phosphorylated at threonine 60. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The WNK [with no lysine (K)] family of serine/threonine kinases is characterized by having a cysteine in place of lysine in subdomain II of its kinase activation domain (1,2). The lysine necessary for phosphoryl transfer is located in an atypical position in the catalytic domain. Four WNK family members have been identified in humans (WNK1-4) and have been implicated in regulating ion permeability (3). Mutations in the WNK1 and WNK4 genes in humans cause pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder leading to hypertension, hyperkalemia, and renal tubular acidosis (4). WNK4 is specifically expressed in the kidney, whereas WNK1 has a wider distribution but is predominantly expressed in polarized epithelia (1-3). Heterozygous mutations in WNK1 in mice result in a significant decrease in blood pressure, while homozygous mutations are embryonic lethal (5). WNK1 is phosphorylated by Akt at Thr60 (6). In addition, WNK1 may be autophosphorylated at Ser382 in the activation loop, and this is thought to be required for its kinase activity (7). |
| 存放说明 | -20C |
| 计算分子量 | 230 |
| 参考文献 | 1 . Veríssimo, F. and Jordan, P. (2001) Oncogene 20, 5562-9. 2 . Xu, B. et al. (2000) J Biol Chem 275, 16795-801. 3 . Choate, K.A. et al. (2003) Proc Natl Acad Sci U S A 100, 663-8. 4 . Wilson, F.H. et al. (2001) Science 293, 1107-12. 5 . Zambrowicz, B.P. et al. (2003) Proc Natl Acad Sci U S A 100, 14109-14. 6 . Vitari, A.C. et al. (2004) Biochem J 378, 257-68. 7 . Xu, B.E. et al. (2002) J Biol Chem 277, 48456-62. |
Western blot analysis of extracts from HT29 cells, untreated or IGF1-treated (100 ng/ml for 30 minutes), using Phospho-WNK1 (Thr60) Antibody (upper) or WNK1 Antibody #4979 (lower). Weatern blot 分析HT29细胞提取物，未刺激组和IGF1 (100 ng/ml ，30 分钟）处理组，所用抗体为Phospho-WNK1 (Thr60) Antibody (上)和WNK1 Antibody #4979 (下). |
