| 货号 | 3877S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Phospho-Vimentin (Ser56) Antibody detects endogenous levels of vimentin only when phosphorylated at Ser56. |
| 使用方法 | WB(1:1000) IF-IC (1:25) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are distinguished by their cell specific expression: cytokeratins (epithelial cells), glial fibrillary acidic protein (GFAP) (glial cells), desmin (skeletal, visceral, and certain vascular smooth muscle cells), vimentin (mesenchyme origin), and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). Vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two (3). Vimentins dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways (4). Phosphorylation of vimentin at Ser56 in smooth muscle cells regulates the structural arrangement of vimentin filaments in response to serotonin (5,6). Remodeling of vimentin and other intermediate filaments is important during lymphocyte adhesion and migration through the endothelium (7). |
| 存放说明 | -20C |
| 计算分子量 | 57 |
| 参考文献 | 1 . Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51. 2 . Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93. 3 . Leader, M. et al. (1987) Histopathology 11, 63-72. 4 . Helfand, B.T. et al. (2004) J Cell Sci 117, 133-41. 5 . Tang, D.D. et al. (2005) Biochem J 388, 773-83. 6 . Fomina, I.G. et al. (1990) Klin Med (Mosk) 68, 125-7. 7 . Nieminen, M. et al. (2006) Nat Cell Biol 8, 156-62. 8 . Yamaguchi, T. et al. (2005) J Cell Biol 171, 431-6. 9 . Oguri, T. et al. (2006) Genes Cells 11, 531-40. 10 . Zhu, Q.S. et al. (2011) Oncogene 30, 457-70. 11 . Xue, G. and Hemmings, B.A. (2013) J Natl Cancer Inst 105, 393-404. 12 . Yamaguchi, T. et al. (2005) J Cell Biol 171, 431-6. 13 . Oguri, T. et al. (2006) Genes Cells 11, 531-40. |
Confocal immunofluorescent analysis of HeLa cells using Phospho-Vimentin (Ser56) Antibody (green) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Phospho-Vimentin (Ser56) Antibody (绿色)和Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (红色)标记，共聚焦免疫荧光分析HeLa细胞。蓝色= DRAQ5® #4084 (DNA荧光染料)。 | |
Western blot analysis of extracts from various cell types, hydroxyurea-treated (4 mM) (G1/S) or paclitaxel-treated (100 nM) (G2/M) for 20 hours, using Phospho-Vimentin (Ser56) Antibody (upper). β-Actin Antibody #4967 was used as a loading control (lower). |
