Phospho-VEGF Receptor 2 (Tyr951) Antibody【科瑞奥博】

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Phospho-VEGF Receptor 2 (Tyr951) Antibody

货号： 2471S 基本售价： 3980.0 元规格： -

产品信息

概述
货号	2471S
反应种属	Human/Mouse
来源宿主	Rabbit
应用	W
目标/特异性	Phospho-VEGF Receptor 2 (Tyr951) Antibody detects endogenous VEGFR-2 only when phosphorylated at Tyr951.
使用方法	WB(1:1000)

性能
供应商	CST
灵敏度	Endogenous
背景	Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).
存放说明	-20C
计算分子量	230
参考文献	1 . Meyer, M. et al. (1999) EMBO J 18, 363-74. 2 . Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38. 3 . Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7. 4 . Takahashi, T. et al. (2001) EMBO J 20, 2768-78. 5 . Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75. 6 . Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605. 7 . Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92. 8 . Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

参考图片

Western blot analysis of extracts of CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domains (Rahimi, N. et al. [2000] J. Biol. Chem. 275, 16986-16992), using Phospho-VEGF Receptor 2 (Tyr951) Antibody (upper) or VEGF receptor 2 antibody (lower).用Phospho-VEGF Receptor 2 (Tyr951) Antibody (上) 或VEGF receptor 2 antibody (下).对含有人CSF-1胞外结合区/小鼠VEGF receptor 2胞内区的嵌合受体的CKR/PAE细胞的提取物进行免疫印迹检测(Rahimi, N. et al. [2000] J. Biol. Chem. 275, 16986-16992)。