| 货号 | 6888S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-ULK1 (Ser757) Antibody recognizes endogenous levels of ULK1 protein only when phosphorylated at Ser757. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Two related serine/threonine kinases, UNC-51-like kinase -1 and -2 (ULK1, ULK2), were discovered as mammalian homologs of the C. elegans gene UNC-51 in which mutants exhibited abnormal axonal extension and growth (1-4). Both proteins are widely expressed and contain an amino-terminal kinase domain followed by a central proline/serine rich domain and a highly conserved carboxy-terminal domain. The roles of ULK1 and ULK2 in axon growth have been linked to studies showing that the kinases are localized to neuronal growth cones and are involved in endocytosis of critical growth factors such as NGF (5). Yeast two-hybrid studies found ULK1/2 associated with modulators of the endocytic pathway, SynGap, and syntenin (6). Structural similarity of ULK1/2 has also been recognized with the yeast autophagy protein Atg1/Apg1 (7). Knockdown experiments using siRNA demonstrated that ULK1 is essential for autophagy (8), a catabolic process for the degradation of bulk cytoplasmic contents (9,10). It appears that Atg1/ULK1 can act as a convergence point for multiple signals that control autophagy (11), and can bind to several autophagy-related (Atg) proteins, regulating phosphorylation states and protein trafficking (12-16). |
| 存放说明 | -20C |
| 计算分子量 | 140-150 |
| 参考文献 | 1 . Ogura, K. et al. (1994) Genes Dev 8, 2389-400. 2 . Kuroyanagi, H. et al. (1998) Genomics 51, 76-85. 3 . Yan, J. et al. (1998) Biochem Biophys Res Commun 246, 222-7. 4 . Yan, J. et al. (1999) Oncogene 18, 5850-9. 5 . Zhou, X. et al. (2007) Proc Natl Acad Sci USA 104, 5842-7. 6 . Tomoda, T. et al. (2004) Genes Dev 18, 541-58. 7 . Matsuura, A. et al. (1997) Gene 192, 245-50. 8 . Chan, E.Y. et al. (2007) J Biol Chem 282, 25464-74. 9 . Reggiori, F. and Klionsky, D.J. (2002) Eukaryot. Cell 1, 11-21. 10 . Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18. 11 . Stephan, J.S. and Herman, P.K. (2006) Autophagy 2, 146-8. 12 . Okazaki, N. et al. (2000) Brain Res Mol Brain Res 85, 1-12. 13 . Young, A.R. et al. (2006) J Cell Sci 119, 3888-900. 14 . Kamada, Y. et al. (2000) J Cell Biol 150, 1507-13. 15 . Lee, S.B. et al. (2007) EMBO Rep 8, 360-5. 16 . Hara, T. et al. (2008) J Cell Biol 181, 497-510. 17 . Kim, J. et al. (2011) Nat Cell Biol 13, 132-41. 18 . Egan, D.F. et al. (2011) Science 331, 456-61. |
Western blot analysis of extracts from A-431 cells, untreated or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 30 min) using Phospho-ULK1 (Ser757) Antibody (upper), or α-Tubulin (11H10) Rabbit mAb #2125 (lower). Western blot 分析A-431细胞的细胞提取物，未处理或人表皮生长因子 (hEGF) #8916 处理 (100 ng/ml, 30 min) ，使用抗体是Phospho-ULK1 (Ser757) Antibody (上图)或 α-Tubulin (11H10) Rabbit mAb 兔单抗#2125 (下图). | |
Western blot analysis of extracts from A172 cells, untreated (-), Torin 1-treated (+; 250 nM; 5 hrs), or INK-128-treated (+; 250 nM; 5 hrs) using Phospho-ULK1 (Ser757) Antibody (upper) or total ULK1 (D8H5) Rabbit mAb #8054 (lower). |
