| 货号 | 3616S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Tuberin/TSC2 (Ser1254) Antibody detects endogenous levels of tuberin only when phosphorylated at serine 1254. This antibody does not detect tuberin phosphorylated at other sites. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).p38-activated kinase MK2 (MAPKAPK-2) phosphorylates Ser1254 of tuberin, and thus augments the interaction between tuberin and 14-3-3 (8). |
| 存放说明 | -20C |
| 计算分子量 | 200 |
| 参考文献 | 1 . Soucek, T. et al. (1998) Proc Natl Acad Sci U S A 95, 15653-8. 2 . Sparagana, S.P. and Roach, E.S. (2000) Curr Opin Neurol 13, 115-9. 3 . Manning, B.D. et al. (2002) Mol Cell 10, 151-62. 4 . Aicher, L.D. et al. (2001) J Biol Chem 276, 21017-21. 5 . Dan, H.C. et al. (2002) J Biol Chem 277, 35364-70. 6 . Goncharova, E.A. et al. (2002) J Biol Chem 277, 30958-67. 7 . Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57. 8 . Li, Y. et al. (2003) J. Biol. Chem. 278, 13663-13671. |
Western blot analysis of extracts from CAD and PC12 cells, using Phospho-Tuberin/TSC2 (Ser1254) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after Western transfer. 应用Phospho-Tuberin/TSC2 (Ser1254) 抗体，Western blot 分析CAD 和PC12细胞提取物。该抗体磷酸化特异性是通过Western转染后，加入（+）和不加入（—）小牛肠碱性磷酸酶（CIP）对膜的处理得到验证。 |
