| 货号 | 13608S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-TTF-1 (Ser327) Antibody recognizes endogenous levels of TTF-1 protein only when phosphorylated at Ser327. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Thyroid Transcription Factor 1 (TTF-1, also known as NKX2-1), a member of the NKX homeobox transcription factor family, was initially discovered in the FRTL-5 rat thyroid cell line (1). Subsequent studies have shown that TTF-1 plays an important role in differentiation and morphogenesis of the developing thyroid, lung, and ventral forebrain (2). TTF-1 controls the expression of several genes, some of which are tissue specific, such as: thyroglobulin, thyroperoxidase, and the thyrotropin receptor in the thyroid; and surfactant proteins and clara cell secretory protein in the lung (2,3). Investigators have found that TTF-1 is expressed in malignant tumors of the thyroid and lung, and it is commonly used as a marker for both primary and malignant lung cancers (4-6). |
| 存放说明 | -20C |
| 计算分子量 | 42 |
| 参考文献 | 1 . Civitareale, D. et al. (1989) EMBO J 8, 2537-42. 2 . Boggaram, V. (2009) Clin Sci (Lond) 116, 27-35. 3 . Yamaguchi, T. et al. (2012) Cancer Cell 21, 348-61. 4 . Whithaus, K. et al. (2012) Arch Pathol Lab Med 136, 155-62. 5 . Yoshida, A. et al. (2011) Lung Cancer 72, 309-15. 6 . Moldvay, J. et al. (2004) Pathol Oncol Res 10, 85-8. 7 . Missero, C. et al. (2000) Mol Cell Biol 20, 2783-93. 8 . Cobellis, G. et al. (1998) Oncogene 17, 2047-57. 9 . Kupperman, E. et al. (1996) Endocrinology 137, 96-104. |
Western blot analysis of extracts from NCI-H3255 cells, untreated (-) or λ phosphatase-treated (+), using Phospho-TTF-1 (Ser327) Rabbit Antibody (upper), Thyroid Transcription Factor 1 (D2E8) Rabbit mAb #12373 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from various cell lines using Phospho-TTF-1 (Ser327) Antibody (upper) and β-Actin (D8A7) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of phospho-TTF-1 (Ser327) from NCI-H3255 cell extracts using Normal Rabbit IgG #2729 (lane 2) or Phospho-TTF-1 (Ser327) Antibody (lane 3). Lane 1 is 10% input. Western blot analysis performed using Phospho-TTF-1 (Ser327) Antibody. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody. |
