| 货号 | 4004S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Troponin I (Cardiac) (Ser23/24) Antibody detects endogenous levels of cardiac troponin I only when phosphorylated at Ser23/24.This antibody does not cross-react with phosphorylated skeletal muscle troponin I. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca2+ sensitivity by reducing the Ca2+ binding affinity of TnC (1-3). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
| 参考文献 | 1 . Ward, D.G. et al. (2002) J Biol Chem 277, 41795-801. 2 . Noland, T.A. et al. (1995) J. Biol. Chem. 270, 25445-25454. 3 . Gaponenko, V. et al. (1999) J. Biol. Chem. 274, 16681-16684. |
Western blot analysis of extracts from mouse myocytes, untreated or stimulated with isoproterenol, using Phospho-Troponin I (Cardiac) (Ser23/24) Antibody (upper) or Troponin I Antibody #4002 (lower). 使用Phospho-Troponin I (Cardiac) (Ser23/24) Antibody (上图)或Troponin I Antibody #4002 (下图)，免疫印迹（Western Blot）分析小鼠肌细胞，细胞分为untreated和isoproterenol处理组。 |
