| 货号 | 4491S |
| 反应种属 | Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Tpl2 (Ser400) Antibody detects transfected Tpl2 only when phosphorylated at Ser400. This antibody does not cross-react with other phosphorylated MAP kinase kinase kinases. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Transfected Only |
| 背景 | Tpl2 (tumor progression locus 2), also known as COT (cancer osaka thyroid), is a serine/threonine kinase expressed primarily in hematopoietic tissues, lung and liver (1). Over-expression of Tpl2 potentiates MAP kinase pathways through MEK1 and SEK1, as well as through MKK6 and MEK5 (2,3). Tpl2 is also engaged in NF-κB activation through NF-κB inducing kinase (NIK), or by inducing phosphorylation and degradation of the NF-κB precusor, p105 NF-κB1 (4,5). Ser400 of Tpl2 is phosphorylated in an Akt-dependent manner. This phosphorylation is required for Tpl2-induced NF-κB-dependent transcription (6). Tpl2 also activates caspase-3 by promoting the assembly of a protein complex of Apaf1 (apoptotic protease-activating factor 1), caspase-9, Tpl2, adaptor protein Tvl1 and procaspase-3 (7). |
| 存放说明 | -20C |
| 计算分子量 | 60, 62 |
| 参考文献 | 1 . Patriotis, C. et al. (1993) Proc Natl Acad Sci U S A 90, 2251-5. 2 . Salmeron, A. et al. (1996) EMBO J 15, 817-26. 3 . Chiariello, M. et al. (2000) Mol Cell Biol 20, 1747-58. 4 . Lin, X. et al. (1999) Immunity 10, 271-80. 5 . Belich, M.P. et al. (1999) Nature 397, 363-8. 6 . Kane, L.P. et al. (2002) Mol Cell Biol 22, 5962-74. 7 . Patriotis, C. et al. (2001) J Cell Physiol 187, 176-87. |
Western blot analysis of extracts from COS cells, untransfected or transfected with rat Tpl2 , using Phospho-Tpl2 (Ser400) Antibody (upper) and Tpl2 Antibody #4492 (lower). In lane 3, Tpl2 transfected COS cell lysate was treated with λ phosphatase to demonstrate the phospho-specificity of Phospho-Tpl2 (Ser400) Antibody.Western blot方法检测细胞提取物：未处理和转染大鼠Tpl2蛋白的COS细胞。使用的抗体是Phospho-Tpl2 (Ser400) Antibody (上图) 和Tpl2 Antibody #4492 (下图). |
