Phospho-TORC1/CRTC1 (Ser151) Antibody【科瑞奥博】

产品中心

当前位置：首页>产品中心

Phospho-TORC1/CRTC1 (Ser151) Antibody

货号： 3359S 基本售价： 3980.0 元规格： -

产品信息

概述
货号	3359S
反应种属	Human
来源宿主	Rabbit
应用	W
目标/特异性	Phospho-Torc1/Crtc1 (Ser151) Antibody detects transfected levels of Torc1/Crtc1 protein when phosphorylated on Ser151.
使用方法	WB(1:1000)

性能
供应商	CST
灵敏度	Transfected Only
背景	Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic β-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes β-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling (1). The protein Torc2 (transducer of regulated CREB activity 2) functions as a CREB co-activator (2,3) and is implicated in mediating the effects of these two pathways (4). In quiescent cells, Torc2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its dissociation from 14-3-3 proteins. Dephosphorylated Torc2 enters the nucleus to promote CREB-dependent transcription. Torc2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting (5). Torc2-related proteins Torc1 and Torc3 also act as CREB co-activators (2,3). Torc1, Torc2 and Torc3 associate with the HTLV Tax protein to promote Tax-dependent transcription of HTLV-1 long terminal repeats (6,7). Torc1 is highly phosphorylated at Ser151 in mouse hypothalamic cells under basal conditions (8). When these cells are exposed to cAMP or a calcium activator, Torc1 is dephosphorylated and translocates into the nucleus (8). Torc1 is essential for energy balance and fertility (8).
存放说明	-20C
计算分子量	82
参考文献	1 . Hinke, S.A. et al. (2004) J Physiol 558, 369-80. 2 . Conkright, M.D. et al. (2003) Mol Cell 12, 413-23. 3 . Iourgenko, V. et al. (2003) Proc Natl Acad Sci U S A 100, 12147-52. 4 . Screaton, R.A. et al. (2004) Cell 119, 61-74. 5 . Koo, S.H. et al. (2005) Nature 437, 1109-11. 6 . Koga, H. et al. (2004) J Biol Chem 279, 52978-83. 7 . Siu, Y.T. et al. (2006) J Virol 80, 7052-9. 8 . Altarejos, J.Y. et al. (2008) Nat Med 14, 1112-7.

参考图片

Western blot analysis of extracts from U-87 MG cells, untransfected or transfected with human wild-type (WT) Torc1 or Torc1 (Ser151Ala), using Phospho-Torc1/Crtc1 (Ser151) Antibody (upper) or DYKDDDDK Tag Antibody (Binds to the same epitope as Sigmas Anti-FLAG® M2 Antibody) #2368 (lower). DYKDDDDK-tagged WT and mutant Torc1 expression vectors were kindly provided by Dr. Marc Montminy at the Salk Institute for Biological Studies.

western blot检测U-87 MG细胞提取物，细胞未转染或用人野生型Torc1或Torc1 (Ser151Ala)转染，所用抗体为Phospho-Torc1/Crtc1 (Ser151) Antibody (上) 和 DYKDDDDK Tag Antibody (Binds to the same epitope as Sigmas Anti-FLAG® M2 Antibody) #2368 (下)。DYKDDDDK-标签野生型及突变型Torc1表达载体由Salk生物学研究所Marc Montminy博士馈赠。