| 货号 | 4226S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Tie2 (Ser1119) Antibody detects transfected levels of Tie2 protein only when phosphorylated at serine 1119. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Transfected Only |
| 背景 | Tie2/Tek is a receptor tyrosine kinase (RTK) expressed almost exclusively on endothelial cells. It is critical for vasculogenesis and could be important for maintaining endothelial cell survival and integrity in adult blood vessels as well as tumor angiogenesis (1-3). A family of ligands known as the angiopoietins binds to Tie2. Interestingly, these ligands appear to have opposing actions, as Angiopoietin-1 (Ang1) and Angiopoietin-4 (Ang4) stimulate tyrosine phosphorylation of Tie2, whereas Angiopoietin-2 (Ang2) and Angiopoietin-3 (Ang3) can inhibit this phosphorylation (4,5). Downstream signaling components, including Grb2, Grb7, Grb14, Shp2, the p85 subunit of phosphatidylinositol 3-kinase and p56/Dok-2, interact with Tie2 in a phosphotyrosine-dependent manner through their SH2 or PTB domains (6,7). Tyr992 is located on the putative activation loop of Tie2 and is a major autophosphorylation site (8). |
| 存放说明 | -20C |
| 计算分子量 | 160 |
| 参考文献 | 1 . Ward, N.L. and Dumont, D.J. (2002) Semin. Cell Dev. Biol. 13, 19-27. 2 . Jones, N. and Dumont, D.J. (2000) Cancer Metastasis Rev. 19, 13-17. 3 . Partanen, J. and Dumont, D.J. (1999) Curr. Top. Microbiol. Immunol. 237, 159-172. 4 . Ellis, L. M. et al. (2002) Oncology 16, 31-35. 5 . Koh, G. Y. et al. (2002) Exp. Mol. Med. 34, 1-11. 6 . Jones, N. et al. (1999) J. Biol. Chem. 274, 30896-30905. 7 . Jones, N. et al. (2003) Mol. Cell. Biol. 23, 2658-2668. 8 . Murray, B. W. et al. (2001) Biochem. 40, 10243-10253. |
Western blot analysis of extracts from Sf9 cells overexpressing GST-human Tie2 kinase domain fusion proteins, wild-type (lane 1) S1119D mutant (lane 2), S1119A mutant (lane 3) or kinase-dead (lane 4) , using Phospho-Tie2 (Ser1119) Antibody (upper) or GST antibody (lower). The wild-type Tie2 kinase domain is constitutively phosphorylated when overexpressed in Sf9 cells. The putative molecular weight of GST-Tie2 fusion is approximately 65 kDa.用Phospho-Tie2 (Ser1119) Antibody (上)或GST antibody (下)对过表达GST-人Tie分子激酶区融合蛋白的Sf9细胞提取物进行免疫印迹检测， 野生型（第一道），S1119D突变（第二道），S1119A突变（第三道）。野生型Tie2激酶区在高表达该蛋白的Sf9细胞中被组成性地磷酸化。GST-Tie2融合蛋白的分子量约为65 kDa。 |
