| 货号 | 2351S |
| 反应种属 | Human/Mouse/Rat/Monkey/All |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/E-P |
| 目标/特异性 | Phospho-Threonine-X-Arginine Antibody detects endogenous levels of proteins containing the phospho-Thr-X-Arg motif. This antibody detects phosphorylated Thr followed by Arg or Lys at the +2 position, though its reactivity is lower for Lys compared to Arg at the +2 position. The antibody does not cross-react with nonphospho-Thr or phospho-Ser in the same motif. It recognizes phospho-Thr in the FFT*R motif in PKCbeta II but does not recognize phospho-Thr in other motifs that lack Lys or Arg at +2. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) IHC-P (1:800) E-P (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Some signaling molecules can be regulated by phosphorylation at a specific threonine followed by arginine or lysine at the +2 position. For example, conventional PKC isozymes phosphorylate substrates containing serine or threonine with Arg or Lys at the -3, -2 and +2 positions (1-2). c-Raf, a mitogen-activated protein kinase and the main effector recruited by GTP-bound Ras, is phosphorylated at Thr481 and Thr491 followed by Lys at the +2 position (3). Phosphorylation of these sites is important for enzyme activities. To determine the phosphorylation state of Thr in the Thr-X-Arg motif, and to identify potential new phosphorylation sites with this motif, Cell Signaling Technology has developed a Phospho-Threonine X-Arginine Antibody that recognizes phosphorylated Thr followed by Arg or Lys at the +2 position. |
| 存放说明 | -20C |
| 参考文献 | 1 . Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60. 2 . Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81. 3 . Zhang BH and Guan KL (2000) EMBO J 19, 5429–39 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing staining of proteins containing phosphorylated threonine-X-arginine motifs, using Phospho-Threonine-X-Arginine Antibody. 对石蜡包埋的人乳腺癌使用Phospho-Threonine-X-Arginine Antibody进行免疫组化分析，显示包含苏氨酸-X-精氨酸模体的蛋白染色。 | |
Phospho-Threonine-X-Arginine Antibody ELISA: Signal-to-noise ratio of phospho- versus nonphospho-peptides containing the phospho-threonine-X-arginine motif. (T* denotes phosphorylated threonine.) Phospho-Threonine-X-Arginine Antibody ELISA分析：包含苏氨酸-X-精氨酸模体的磷酸化和非磷酸化的信噪比。（T*代表磷酸化的苏氨酸） | |
Western blot analysis of extracts from Jurkat cells, untreated, TPA-treated or calyculin A-treated, using Phospho-Threonine-X-Arginine Antibody. 对Jurkat细胞，未处理或TPA或calyculin A处理，使用Phospho-Threonine-X-Arginine Antibody进行Western blot分析。 |
