Phospho-TBC1D1 (Ser660) Antibody
货号：
6928S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 6928S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-TBC1D1 (Ser660) Antibody recognizes transfected levels of TBC1D1 protein only when phosphorylated at Ser660. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | TBC1D1 is a paralog of AS160 (1) and both proteins share about 50% identity (2). TBC1D1 was shown to be a candidate gene for severe obesity (3). It plays a role in Glut4 translocation through its GAP activity (2,4). Studies indicate that TBC1D1 is highly expressed in skeletal muscle (1). Insulin, AICAR, and contraction directly regulate TBC1D1 phosphorylation in this tissue (1). Three AMPK phosphorylation sites (Ser231, Ser660, and Ser700) and one Akt phosphorylation site (Thr590) were identified (5). Muscle contraction or AICAR treatment increases phosphorylation on Ser231, Ser660, and Ser700 but not on Thr590; insulin increases phosphorylation on Thr590 only (5). |
| 存放说明 | -20C |
| 计算分子量 | 160 |
参考图片Western blot analysis of extracts from 293T cells, transfected with either mouse wild-type TBC1D1 or mutant TBC1D1 (S660A), using Phospho-TBC1D1 (Ser660) Antibody (upper) or TBC1D1 (G689) Antibody #5929 (lower). Both expression vectors were kindly provided by Dr. Laurie Goodyear at the Joslin Diabetes Center. Western blot检测293T细胞提取物，细胞用鼠野生型TBC1D1和突变TBC1D1(S660A)进行了转染，所用抗体为Phospho-TBC1D1 (Ser660) Antibody（上）和TBC1D1 (G689) Antibody #5929（下）。两个表达载体都由Joslin糖尿病中心的Laurie Goodyear博士馈赠。 |
