| 货号 | 9339S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2). |
| 存放说明 | -20C |
| 计算分子量 | 82 |
Western blot analysis of extracts from K562 (human), C2C12 (mouse) and C6 (rat) cell lines, using Phospho-TAK1 (Ser412) Antibody.western blot方法检测细胞提取物：K562 (人源), C2C12 (小鼠) 和C6 (大鼠)细胞系。使用的抗体是Phospho-TAK1 (Ser412) Antibody。 | |
Western blot analysis of extracts from HeLa cells co-treated with and without 100nM Calyculin A (#9902) and 20 ng/mL hIL-1β (#8900) for 10 min., and transfected with and without 100nM SignalSilence®TAK1 siRNA I (#6317) using Phospho-TAK1 (Ser412) Antibody #9339 (top) and β-actin (13E5) Rabbit mAb #4970 (bottom). |
