Phospho-Stathmin (Ser38) Antibody
货号：
3426S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3426S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Stathmin (Ser38) Antibody detects endogenous levels of stathmin protein only when phosphorylated at Ser38. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Stathmin is a ubiquitously expressed microtubule destabilizing phosphoprotein that is upregulated in a number of cancers. The amino terminus of the protein contains multiple phosphorylation sites and is involved in the promotion of tubulin filament depolymerization. Phosphorylation at these sites inactivates the protein and stabilizes microtubules. Ser16 phosphorylation by CaM kinases II and IV (1,2) increases during G2/M-phase and is involved in mitotic spindle regulation (3,4). Ser38 is a target for cdc2 kinase (5) and TNF-induced cell death gives rise to reactive oxygen intermediates leading to hyperphosphorylation of stathmin (6). EGF receptor activation of Rac and cdc42 also increases phosphorylation of stathmin on Ser16 and Ser38 (7). Other closely related family members are neuronally expressed and include SCG10, SCLIP, RB3 and its splice variants RB3 and RB3. Stathmin and SCG10 have been shown to play roles in neuronal-like development in PC12 cells (8). |
| 存放说明 | -20C |
| 计算分子量 | 19, 20 |
参考图片Western blot analysis of extracts from HT-29 cells, untreated or treated with nocodazole for 16 hours at 100 ng/mL and unblocked (left), blocked with phospho-stathmin (Ser38) peptide (middle) or phospho-stathmin (Ser16) peptide (right), using Phospho-Stathmin (Ser38) Antibody. 使用Phospho-Stathmin (Ser38) Antibody，免疫印迹（Western Blot）分析HT-29细胞中Phospho-Stathmin蛋白水平。细胞分为untreated或100 ng/mL nocodazole-treated（16小时），抗体分别孵育unblocked (左图)、phospho-stathmin (Ser38) peptide (中图)或phospho-stathmin (Ser16) peptide (右图)。 |
