| 货号 | 13873S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Slingshot homolog 3 (SSH3) is a protein phosphatase that modifies actin cytoskeleton dynamics via cofilin dephosphorylation. Cofilin is an evolutionarily conserved, actin-binding protein that severs actin filaments during processes that rely on actin filament dynamics, including cytokinesis, cell migration, invasion, and neuronal development. Actin severing and filament depolymerization are regulated through the controlled cycling of cofilin between the phosphorylated and dephosphorylated forms (1). The kinases LIMK and TESK inactivate cofilin by phosphorylating it at Ser3 (2,3). The slingshot homologs (SSH1, SSH2, and, to a lesser extent, SSH3) and chronophin/PDXP phosphatases remove phosphate from cofilin at Ser3, enabling cofilin binding to actin and filament depolymerization (3). SSH3 is widely expressed in epithelial tissues, and has been found to be non-essential for viability and fertility in knockout mice (4). While its biological function remains elusive, phosphorylation at Ser37 of SSH3 has been identified in several phosphoproteomic studies (5-7). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Western blot analysis of extracts from ZR-75-1 cells, untreated or λ phosphatase and CIP-treated, using Phospho-SSH3 (Ser37) Antibody (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Western blot analysis of extracts from various cell lines, untreated or Calyculin A #9902-treated, using Phospho-SSH3 (Ser37) Antibody (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Western blot analysis of extracts from 293T cells, untransfected or transfected with a construct expressing Myc/DDK-tagged full-length human SSH3 protein, using Phospho-SSH3 (Ser37) Antibody (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower). |
