| 货号 | 2979S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 使用方法 | WB(1:1000) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | There are three members of the steroid receptor co-activator (SRC) family of proteins: SRC-1 (NCoA-1), SRC-2 (TIF2/GRIP1/NCoA-2) and SRC-3 (ACTR/pCIP/RAC3/TRAM-1/AIB1). The SRC family members all share significant structural homology and function in a similar fashion to stimulate transcription mediated by nuclear hormone receptors and other transcriptional activators such as STAT3, NF-κB, E2F1 and p53 (1-4). Two SRC proteins, SRC-1 and SRC-3, function as histone acetyltransferases (5,6). In addition, all three family members can recruit other histone acetyltransferases (CBP/p300, PCAF) and histone methyltransferases (PRMT1, CARM1) to target promoters and cooperate to enhance expression of many genes (5-8). The SRC proteins play important roles in multiple physiological processes including cell proliferation, cell survival, somatic cell growth, mammary gland development, female reproductive function and vasoprotection (9). SRC-1 and SRC-3 are conduits for kinase-mediated growth factor signaling to the estrogen receptor and other transcriptional activators. Seven SRC-1 phosphorylation sites and six SRC-3 phosphorylation sites have been identified, which are induced by steroids, cytokines and growth factors and involve multiple kinase signaling pathways (9-11). All three SRC family members are associated with increased activity of nuclear receptors in breast, prostate and ovarian carcinomas. In addition, SRC-3 is frequently amplified or over-expressed in a number of cancers (12), and SRC-1/PAX3 and SRC-2/MYST3 translocations are found associated with rhabdomyosarcoma and acute myeloid leukemia, respectively (13,14). |
| 存放说明 | -20C |
| 计算分子量 | 160 |
Western blot analysis of cell extracts from MCF-7 cells, untreated or stimulated with heregulin, using Phospho-SRC-3 (Thr24) Antibody (upper and middle) or SRC-3 (5E11) Rabbit mAb #2126 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. 使用Phospho-SRC-3 (Thr24) Antibody (上图和中图)或SRC-3 (5E11) Rabbit mAb #2126 (下图)，免疫印迹(Western blot)分析MCF-7细胞中Phospho-SRC-3 (Thr24)和SRC-3 (5E11)的蛋白水平。中图的免疫印迹实验在加入抗体之前使用calf intestinal phosphatase (CIP)处理。 | |
Confocal immunofluorescent analysis of HeLa cells, either untreated (left) or λ-phosphatase treated (right), using Phospho-SRC-3 (Thr24) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用Phospho-SRC-3 (Thr24) Antibody (红色)标记，共聚焦免疫荧光分析HeLa细胞，细胞分为untreated (左图)或λ-phosphatase treated (右图)。DY-554 phalloidin标记微丝蛋白。 |
